Productivity refers to the maximum use of human resources in service provision. Due to the existence of advanced equipment and instruments for surgery, the operating room is one of the most complex and income-generating sections of a hospital. Since the improvement of operating room nurses’ productivity can lead to enhanced hospital productivity, this study was done to determine the productivity rate of operating room nurses in Iran. This cross-sectional study was done on 533 operating room nurses from 8 metropolises in Iran in 2022. The sampling method in this research was multistage. The data collection instruments were Hersy & Belanchard & Gold.Smith productivity questionnaire and demographic characteristics. After receiving the ethics code, data collection was done for three months. Descriptive and inferential analyses, including independent t-tests and analysis of variance, were used for data analysis. The collected data were analyzed by SPSS 18. P-value < 0.05 was considered statistically significant. The mean age of the participants was 29.83 ± 5.94 and 421 of the participants were women. The mean and SD for total productivity in operating room nurses was obtained at 60.83 ± 12.78, below average. Data analysis shows that total productivity was significantly associated with marital status (P-value = 0.035) and working experience (P-value = 0.019). Considering the low mean productivity rate of operating room nurses, researchers recommend that hospital managers pay more attention to the productivity of operating room nurses by knowing the influential factors and implementing proper and high-quality management.