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Surface Modification of Poly(methyl methacrylate) Used in the Fabrication of Microanalytical DevicesHenry, Alyssa C; Tutt, Tumari J; Galloway, Michelle; Davidson, Yolanda Y; McWhorter, C. Scott; Soper, Steven A; McCarley, Robin L
Analytical chemistry (Washington), 11/2000, Letnik: 72, Številka: 21Journal Article
We report here the chemical modification of poly(methyl methacrylate) (PMMA) surfaces by their reaction with the monoanion of α,ω-diaminoalkanes (aminolysis reaction) to yield amine-terminated PMMA surfaces. It is found that the amine functionalities are tethered to the PMMA backbone through an alkane bridge to amide bonds formed during the aminolysis of the surface ester functionalities. The distribution of the amine termini is quite uniform as judged by fluorescence micrographs. It is found that the electroosmotic flow in aminated PMMA microchannels is reversed when compared to that in unmodified channels. In addition, it is demonstrated that enzymes can be immobilized onto the amine-terminated PMMA surfaces and are effective in the restriction digestion of dsDNAs. Finally, the availability of the surface amine groups is further demonstrated by their reaction with n-octadecane-1-isocyanate to form PMMA surfaces terminated with well-ordered and highly crystalline octadecane chains.
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