The prevalence of liver disease has led to its emergence as a major challenge. This challenge is also attributed to factors such as the challenging diagnosis, complexity of pathogenesis, and the absence of established therapies. When Hepatitis B virus (HBV) infections occurs in patients that do not have detectable hepatitis B surface antigen (HBsAg), it is referred to as occult infections. Despite the fact that these kinds of infections have been found in patients with chronic hepatitis C liver disease, there is still no evidence on their clinical implication and prevalence. HBV is a small partial deoxy ribonucleic acid (DNA) virus with like retroviruses. The virus falls under the group of Hepadnavirus infections and family of orthohepadna virus. About 66% of patients with acute HBV infection have an asymptomatic, mild, and sub-clinical illness that typically goes undetected. Thus, in this work, hematological parameters were used in detecting the virus, and the results of the hematological parameters revealed significant changes in white blood cell (WBC), lymphocyte and platelet area under curve (AUC) (0.95), (0.66), and (0.82). The mean values for ALT and ALP in HBV-positive patients increased significantly as compared to the control. Similarly, there was no significant difference between the AUC, CI for HBV-positivity for ALT 0.91, (0.8485-0.9854) and ALP 0.89 (0.8123-0.9633). This study revealed a significant positive correlation between ALP level and ALT and each lymphocyte, granulocyte and WBCs. Thus, each of them may be considered as major factors of development of HBV level.