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Rabe, Patrick; Rinkel, Jan; Nubbemeyer, Britta; Köllner, Tobias G.; Chen, Feng; Dickschat, Jeroen S.
Angewandte Chemie (International ed.), December 5, 2016, Letnik: 55, Številka: 49Journal Article
Genome sequences of social amoebae reveal the presence of terpene cyclases (TCs) in these organisms. Two TCs from Dictyostelium discoideum converted farnesyl diphosphate into (2S,3R,6S,9S)‐(−)‐protoillud‐7‐ene and (3S)‐(+)‐asterisca‐2(9),6‐diene. The enzyme mechanisms and EI‐MS fragmentations of the products were studied by labeling experiments. Terpene time: The products of two terpene cyclases from the social amoeba Dictyostelium discoideum were identified as (2S,3R,6S,9S)‐protoillud‐7‐ene and (3S)‐asterisca‐2(9),6‐diene. The enzyme mechanisms and the EI‐MS fragmentation reactions of their products were studied in extensive isotopic labeling experiments.
