In this work, a simple synthesis of low-toxicity transition metal material of WO dots was used as a co-reactant with Au@SiO as a core-shell material and a signal amplification factor to collaboratively promote Ru(bpy) electrochemiluminescence (ECL) for the construction of a highly sensitive aptasensor for the detection of diazinon (DZN) in vegetables. Electrodes modified with multi-walled carbon nanotubes-chitosan composite membranes (MWCNTs-CS) were used to load and immobilize more Ru(bpy) .can load more Ru(bpy) . WO dots synthesized by a simple method showed excellent ECL efficiency as a novel co-reactant for Ru(bpy) . Under optimized conditions, this aptasensor for DZN has a wide detection range (10 pg mL - 1 μg mL .) and a low detection limit (0.0197 ng L ). The aptasensor has shown good results in the analysis of real samples in the experiment. This work provides a new approach to the construction of a novel electrochemiluminescence sensor for the detection of pesticides.