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PIYANAN, Thirada; ATHIPORNCHAI, Anan; HENRY, Charles S.; SAMEENOI, Yupaporn
Analytical Sciences, 01/2018, Letnik: 34, Številka: 1Journal Article
This work reports a portable distance-based detection paper device that has a thermometer-like shape for rapid, instrument-free determination of antioxidant activity using a nanoceria assay. The assay is based on partial reduction of cerium ion from Ce4+ to Ce3+ on nanoceria deposited along the detection channel by antioxidants present in food, giving highly reactive oxidation products. Either these products or the parent antioxidant compounds could then bind to the OH-rich ceria nanoparticles and generate charge transfer ceria–antioxidant complexes resulting in a yellow to brown color change. The distance of the brown color on the detection channel is directly proportional to antioxidant activity, and can be easily measured using an integrated ruler without the need of any external sophisticated instrument for detection. The paper sensor has been studied for the analysis of common antioxidants and its performance was validated against traditional antioxidant assays for 11 tea sample analyses. Using the Spearman rank correlation coefficient method, the antioxidant activity of tea samples obtained from the paper device correlated with the traditional assay at the 95% confidence level. The developed sensor provided a high recovery and tolerance limit and was stable for 50 days both when stored at ambient and low temperature (6 and −20°C). The results demonstrated that the developed paper device is an alternative to allow for fast, simple, instrument-free, cheap, portable and high-throughput screening of antioxidant activity analysis in real samples.
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Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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