Real‐time and continuous monitoring of physiological signals is essential for mobile health, which is becoming a popular tool for efficient and convenient medical services. Here, an active pulse sensing system that can detect the weak vibration patterns of the human radial artery is constructed with a sandwich‐structure piezoelectret that has high equivalent piezoelectricity. The high precision and stability of the system result in possible medical assessment applications, including the capability to identify common heart problems (such as arrhythmia); the feasibility to conduct pulse palpation measurements similar to well‐trained doctors in Traditional Chinese Medicine; and the possibility to measure and read blood pressure. A piezoelectret‐based human pulse sensing system with excellent sensitivity and stability is applied for medical assessments. Pulse waves classification, arrhythmia diagnosis, blood pressure measurement, and imitation of three‐finger pulse palpation in Traditional Chinese Medicine are successfully demonstrated, indicating the potential application in wearable mobile health.