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Vauleon, S; Mueller, S
Chembiochem : a European journal of chemical biology, March 3, 2003, Letnik: 4, Številka: 2-3Journal Article
Outside influence: External regulation of hairpin ribozyme derivatives by oligonucleotide effectors offers the possibility of switching activity in a defined way and opens up attractive methods for structure and function analysis. An oligonucleotide effector was used to reactivate two inactive mutants of the hairpin ribozyme (see figure). The effector replaced the mutated sequence of the ribozyme and restored its catalytically competent conformation. The same technique could be used to interrupt and then reactivate the catalytic cycle at specific steps and thus to study the structure and function of other ribozymes.
