Starch-rich raw materials are widely used in the food industry. Their functionality and end-use applications are markedly influenced by starch characteristics. Starches with varying amylose (AM) and amylopectin (AP) content are of particular interest due to their ability to influence and modify the texture, quality and stability of starch-based food products. The present study shows the influence of the AM/AP content on physicochemical and morphological properties of a range of starches (Maize = 3%, 23%, 71%; Potato = 2%, 21%; Wheat = 28; Barley = 3%, 25% AM content w/w of starch). Starches have been analyzed in terms of their chemical composition, water retention capacity, morphological characteristics, and pasting/thermal properties. The changes in starch granule morphology during gelatinization were monitored by confocal laser scanning microscopy (CLSM). The different analysis revealed that waxy-starches (AP>90%) had a high water retention capacity (1.2–1.5 times higher) and developed higher paste viscosities (up to 40% for maize; 43% for barley). The swollen granules were highly susceptible to mechanical breakdown and solubilized faster. Higher AM contents showed inhibition of an extensive granule swelling and lowered the paste viscosity. The exceptional integrity of the high-AM starch even prevented its gelatinization at atmospheric pressure. Significant differences in physicochemical and morphological properties between the starches from regular, high-AM and waxy strains have become evident, no direct relationship between the AM/AP contents and the internal growth ring structures of the starch granules could be identified by CLSM. The waxy starches had a higher gelatinization temperature (up to 2 °C) and enthalpy (up to 20%), which indicates a higher crystalline and molecular order. Display omitted ► Relationships of the AM/AP content and the chemical, visual, pasting, thermal characteristics. ► Gelatinization behavior of starch granules visualized and analyzed by a micrograph processing. ► Morphological characterization by micrographs analyzes of different starches. ► CLSM micrographs combined with pasting properties and thermal changes starches.