Abstract In recent years, with the rapid economic development, people’s spiritual needs are also increasing, and more and more concerts and sports games appear in public life. However, these activities have great potential risks, and the frequent occurrence of security incidents poses a great threat to the safety of the city and the public. How to evacuate quickly and effectively has become an urgent problem to be solved. Studies have shown that during emergency, people show herd behavior during evacuation, and do not necessarily follow evacuation signs. In this paper, through simulated evacuation experiments, combined with eye movement technology, this paper studies the influence of the direction of evacuation signs and disturbing crowd on the validity of evacuation signs during the evacuation process. And the processing and analysis of the experimental data has certain reference significance for the related research on the validity of evacuation signs.