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Koo, Bonhan; Jin, Choong Eun; Park, Se Yoon; Lee, Tae Yoon; Nam, Jeonghun; Jang, Young‐Rock; Kim, Sun Mi; Kim, Ji Yeun; Kim, Sung‐Han; Shin, Yong
Journal of biophotonics, April 2018, 2018-04-00, 20180401, Letnik: 11, Številka: 4Journal Article
Recent zoonotic outbreaks, such as Zika, Middle East respiratory syndrome and Ebola, have highlighted the need for rapid and accurate diagnostic assays that can be used to aid pathogen control. Q fever is a zoonotic disease caused by the transmission of Coxiella burnetii that can cause serious illness in humans through aerosols and is considered a potential bioterrorism agent. However, the existing assays are not suitable for the detection of this pathogen due to its low levels in real samples. We here describe a rapid bio‐optical sensor for the accurate detection of Q fever and validate its clinical utility. By combining a bio‐optical sensor, that transduces the presence of the target DNA based on binding‐induced changes in the refractive index on the waveguide surface in a label‐free and real‐time manner, with isothermal DNA amplification, this new diagnostic tool offers a rapid (<20 min), 1‐step DNA amplification/detection method. We confirmed the clinical sensitivity (>90%) of the bio‐optical sensor by detecting C. burnetii in 11 formalin‐fixed, paraffin‐embedded liver biopsy samples from acute Q fever hepatitis patients and in 16 blood plasma samples from patients in which Q fever is the cause of fever of unknown origin. Schematic representation of the 3 detection methods including the conventional assays and the bio‐optical sensor for formalin‐fixed, paraffin‐embedded tissue and blood plasma samples. The bio‐optical sensor can simultaneously amplify and detect the target DNA in a label‐free and real‐time manner (#1). The conventional assays (end‐point and real‐time PCRs) are composed of DNA amplification and detection step separately (#2 and #3)
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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