Ti-Nb alloys are potential candidates owing to their excellent mechanical properties and high corrosion resistance for bioactive implant applications compared to other metallic materials. The purpose of this work is to investigate the effect of the Nb amount on the microstructure, mechanical properties, corrosion behavior and hydroxyapatite (HA) formation ability of Ti-Nb alloys produced via powder injection molding. According to the phase/microstructure studies, including XRD analyses, optical microscope, and SEM imaging, all of the alloys are composed of α+β phases, and with the increment of Nb content, β phase stability increases. Also, it is observed that Nb content has significant effects on the mechanical properties of the considered alloys. While the hardness/Transverse rupture strength values of Ti-Nb alloys (303-340HV, 992-440 MPa) are higher than that of the titanium (269HV, 300 MPa), the elastic modulus is measured as lower than (100–115 GPa) that of the titanium (132–140 GPa). The increment of Nb content causes to decrease of the hardness, bending strength and elastic modulus of Ti-Nb alloys. Also, the addition of Nb contributed to the improvement of corrosion resistance and induced to increase of hydroxyapatite formation ability. •Nb addition induced to increase of hydroxyapatite formation ability.•Nb addition to the Ti contributed to the improvement of corrosion resistance.•With the increment of Nb content, stability of the β phase increases.•Highest Transverse Rupture Strength value (992 MPa) was obtained from Ti16 Nb alloy.•Lowest Elastic Modulus value was achieved when alloying Ti with 28 % wt. Nb.