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Kuniyoshi, H.(Hiroshima Univ. (Japan)); Fukui, Y; Sakai, Y
Bioscience, biotechnology, and biochemistry, 08/2006, Letnik: 70, Številka: 8Journal Article
We designed a new inverse PCR protocol combined with switching mechanism at 5', end of RNA transcript (SMART) technology, and applied it to the cloning of teleost corticotropin-releasing hormone precursor cDNA. Due to the advantages of both techniques, this method can efficiently amplify the complete 5'- and 3'ends of cDNA in a single reaction, and might prove to be an alternative to the conventional rapid amplification of cDNA ends (RACE) approaches.
