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Xie, Xin‐Xin; Chen, Zhong‐Jian; Zhu, Quan‐Gang; Yu, Qin; Lian, Tian‐Yan; Xu, Xue‐Lian; Chen, Ya; Song, Wei‐Hua
Journal of separation science, January 2024, 2024-Jan, 2024-01-00, 20240101, Letnik: 47, Številka: 1Journal Article
Centrifugal partition chromatography in the pH‐zone‐refining mode was successfully applied to the separation of alkaloids from the crude extract of Corydalis decumbens. The experiment was performed with a two‐phase solvent system composed of petroleum ether‐ethyl acetate‐ethanol‐water (5:5:3:7, v/v/v/v) where triethylamine (10 mM) was added to the stationary phase and hydrochloric acid (10 mM) to the mobile phase. From 1.6 g of the crude extract, 43 mg protopine, 189 mg (+)‐egenine, and 158 mg tetrahydropalmatine were obtained with a purity of 98.2%, 94.6%, and 96.7%, respectively. Tetrahydropalmatine showed an interesting anticomplement effect with CH50 0.11 and AP50 0.25 mg/mL, respectively. In a mechanistic study, tetrahydropalmatine interacted with C1, C3, C4, and C5 components in the complement activation cascade.
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