► Road outside urban area is cognitively less demanding than inside the urban area. ► Curves decrease mean speed but increase SD acceleration/deceleration and SDLP. ► PDT performance indicated higher workload when curves were present. ► Gates locally reduced speed but increased SD of acceleration/deceleration and SDLP. ► Curves and gate constructions can improve traffic safety. This study examined the impact of traffic calming measures (TCM) on major roads in rural and urban areas. More specifically we investigated the effect of gate constructions located at the entrance of the urban area and horizontal curves within the urban area on driving behavior and workload. Forty-six participants completed a 34km test-drive on a driving simulator with eight thoroughfare configurations, i.e., 2 (curves: present, absent)×2 (gates: present, absent)×2 (peripheral detection task (PDT): present, absent) in a within-subject design. PDT performance (mean response time (RT) and hit rate) indicated that drivers experienced the road outside the urban area as cognitively less demanding relative to the more complex road environment inside the urban area. Whereas curves induced a speed reduction that was sustained throughout the entire urban area, variability of acceleration/deceleration and lateral position were increased. In addition, PDT performance indicated higher workload when curves were present (versus absent). Gate constructions locally reduced speed (i.e., shortly before and after the entrance) and slightly increased variability of acceleration/deceleration and lateral position nearby the entrance. However, the effects on SDL-A/D and SDLP are too small to expect traffic safety problems. It can be concluded that both curves and gate constructions can improve traffic safety. Notwithstanding, the decision to implement these measures will depend on contextual factors such as whether the road serves a traffic-, rather than a residential function.