Selection of Sequence elements that substitute for the standard AATAAA motif which signals 3′ processing and polyadenylation of late simian virus 40 mRNAs

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Selection of Sequence elements that substitute for the standard AATAAA motif which signals 3′ processing and polyadenylation of late simian virus 40 mRNAs

Swimmer, Candace; Shenk, Thomas

Nucleic acids research, 11/1985, Letnik: 13, Številka: 22

Journal Article

A method is described which allows selection of sequences which can substitute for the normal AATAAA hexanucleotide involved in polyadenylation of SV40 late mRNAs. Plaques were generated from viral DNA lacking the motif, forcing acquisition of substitute sequences. Four variants wre characterized. All displayed wild-type growth kinetics and produced normal levels of late mRNAs and proteins. Two variants had reacquired AATAAA elements and one acquired an ATTAAA sequence. The last variant carried an ATTTTTTAAA segment, suggesting this novel sequence, or some portion of it, can also signal poly A addition.

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Swimmer, Candace | Shenk, Thomas

Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.

Leto	Faktor vpliva		Izdaja		Kategorija		Razvrstitev
Leto	JCR	SNIP	JCR	SNIP	JCR	SNIP	JCR	SNIP

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