E-viri
Recenzirano
Odprti dostop
-
Cowley, Glenn S; Weir, Barbara A; Vazquez, Francisca; Tamayo, Pablo; Scott, Justine A; Rusin, Scott; East-Seletsky, Alexandra; Ali, Levi D; Gerath, William Fj; Pantel, Sarah E; Lizotte, Patrick H; Jiang, Guozhi; Hsiao, Jessica; Tsherniak, Aviad; Dwinell, Elizabeth; Aoyama, Simon; Okamoto, Michael; Harrington, William; Gelfand, Ellen; Green, Thomas M; Tomko, Mark J; Gopal, Shuba; Wong, Terence C; Wong, Terrence C; Li, Hubo; Howell, Sara; Stransky, Nicolas; Liefeld, Ted; Jang, Dongkeun; Bistline, Jonathan; Hill Meyers, Barbara; Armstrong, Scott A; Anderson, Ken C; Stegmaier, Kimberly; Reich, Michael; Pellman, David; Boehm, Jesse S; Mesirov, Jill P; Golub, Todd R; Root, David E; Hahn, William C
Scientific data, 09/2014, Letnik: 1, Številka: 1Journal Article
Using a genome-scale, lentivirally delivered shRNA library, we performed massively parallel pooled shRNA screens in 216 cancer cell lines to identify genes that are required for cell proliferation and/or viability. Cell line dependencies on 11,000 genes were interrogated by 5 shRNAs per gene. The proliferation effect of each shRNA in each cell line was assessed by transducing a population of 11M cells with one shRNA-virus per cell and determining the relative enrichment or depletion of each of the 54,000 shRNAs after 16 population doublings using Next Generation Sequencing. All the cell lines were screened using standardized conditions to best assess differential genetic dependencies across cell lines. When combined with genomic characterization of these cell lines, this dataset facilitates the linkage of genetic dependencies with specific cellular contexts (e.g., gene mutations or cell lineage). To enable such comparisons, we developed and provided a bioinformatics tool to identify linear and nonlinear correlations between these features.
Avtor
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.