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Yu, Chien-Hsiung; Davidson, Sophia; Harapas, Cassandra R.; Hilton, James B.; Mlodzianoski, Michael J.; Laohamonthonkul, Pawat; Louis, Cynthia; Low, Ronnie Ren Jie; Moecking, Jonas; De Nardo, Dominic; Balka, Katherine R.; Calleja, Dale J.; Moghaddas, Fiona; Ni, Erya; McLean, Catriona A.; Samson, Andre L.; Tyebji, Shiraz; Tonkin, Christopher J.; Bye, Christopher R.; Turner, Bradley J.; Pepin, Genevieve; Gantier, Michael P.; Rogers, Kelly L.; McArthur, Kate; Crouch, Peter J.; Masters, Seth L.
Cell, 10/2020, Letnik: 183, Številka: 3Journal Article
Cytoplasmic accumulation of TDP-43 is a disease hallmark for many cases of amyotrophic lateral sclerosis (ALS), associated with a neuroinflammatory cytokine profile related to upregulation of nuclear factor κB (NF-κB) and type I interferon (IFN) pathways. Here we show that this inflammation is driven by the cytoplasmic DNA sensor cyclic guanosine monophosphate (GMP)-AMP synthase (cGAS) when TDP-43 invades mitochondria and releases DNA via the permeability transition pore. Pharmacologic inhibition or genetic deletion of cGAS and its downstream signaling partner STING prevents upregulation of NF-κB and type I IFN induced by TDP-43 in induced pluripotent stem cell (iPSC)-derived motor neurons and in TDP-43 mutant mice. Finally, we document elevated levels of the specific cGAS signaling metabolite cGAMP in spinal cord samples from patients, which may be a biomarker of mtDNA release and cGAS/STING activation in ALS. Our results identify mtDNA release and cGAS/STING activation as critical determinants of TDP-43-associated pathology and demonstrate the potential for targeting this pathway in ALS. Display omitted •TDP-43 enters mitochondria, triggers mtDNA release via the mPTP•TDP-43-induced cytosolic mtDNA accumulation activates the cGAS/STING pathway•Evidence of cytoplasmic mtDNA was found in ALS patient cells and disease models•Blocking STING prevents inflammation and neurodegeneration in vitro and in vivo TDP-43 causes inflammation in ALS by stimulating mitochondrial DNA release, which is subsequently sensed by the cytosolic cGAS/STING pathway, suggesting that inhibition of cGAS/STING could help alleviate inflammation-related damage in ALS.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Vir: Osebne bibliografije
in: SICRIS
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