Incivility among nurses and matrons can lead to various issues such as employment turnover and dysfunctional patient care. Therefore, the need to examine and assess the uncivil behaviors among nurses and matrons is evident. The main purpose of this article is to study and determine the frequency of incivility between nurses and matrons from nurses’ point of view. In this cross-sectional study, 200 nurses were selected from three educational hospitals of Mashhad using stratified-cluster random sampling method. The main research instrument was the edited Ottinot’s questionnaire of “perceived workplace civility climate scale (PWCC)” as well as general demographic information questionnaire. Data were analyzed using descriptive and inferential statistics in SPSS version 11.5. According to research findings, 22.6% of isolating behaviors, 27% of gossiping behaviors, 28% of hostile behaviors, and 26.5% of privacy violation behaviors were reported among matrons for at least one instance. In total, 28.8% (45 persons) admitted that they had observed at least one instance of uncivil behaviors in workplace once or twice. Incivility exists among a quarter of nurses and matrons. Since in nursing society and culture of Islamic Iran these behaviors cannot be tolerated, it is highly recommended that managers, strategists, and nursing teachers pay a special attention to such behaviors among students, employers, and co-workers Nepristojno ponašanje u međusobnom odnosu medicinskih sestara i glavnih medicinskih sestara može da dovede do mnogih posledica, poput promene radnog mesta i neadekvatne nege bolesnika. Iz tog razloga, potreba za ispitivanjem i procenom nepristojnog ponašanja u međusobnom odnosu medicinskih sestara i glavnih medicinskih sestara je očigledna. Cilj ovog rada bio je da analizira i odredi učestalost nepristojnog ponašanja u odnosu između medicinskih sestara i glavnih medicinskih sestara sa stanovišta medicinskih sestara. Za ovu studiju preseka odabrano je dvesta medicinskih sestara iz triju bolnica u Mashhadu pomoću stratifikovane kluster metode slučajnog uzorka. Osnovni instrument istraživanja bio je prilagođeni Ottinotov upitnik sa skalom za procenu pristojnosti na radnom mestu (PWCC skala), kao i upitnik o opštim demografskim podacima. Podaci su analizirani primenom deskriptivne i inferentne statistike, u SPSS programskom paketu, verzija 11.5. Prema rezultatima istraživanja, u ponašanju glavnih medicinskih sestara identifikovano je 22,6% izolovanog ponašanja, 27% ogovaranja, 28% neprijateljskog ponašanja i 26% narušavanja privatnosti. Ukupno, 28,8% (45 osoba) je priznalo da su na radnom mestu primetili barem jedan oblik nepristojnog ponašanja jednom ili dva puta. Nepristojno ponašanje je zabeleženo kod jedne četvrtine medicinskih sestara i glavnih medicinskih sestara. S obzirom da se u krugovima sestrinstva i islamske zajednice ovi oblici ponašanja ne mogu tolerisati, menadžerima, stratezima i nastavnicima se ozbiljno preporučuje da obrate pažnju na ovakve oblike ponašanja među studentima, zaposlenima i saradnic.