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  • Multicentre study on the re...
    Rodriguez-Temporal, David; Alcaide, Fernando; Mareković, Ivana; O'Connor, James Anthony; Gorton, Rebecca; van Ingen, Jakko; Van den Bossche, An; Héry-Arnaud, Genevieve; Beauruelle, Clémence; Orth-Höller, Dorothea; Palacios-Gutiérrez, Juan-José; Tudó, Griselda; Bou, Germán; Ceyssens, Pieter-Jan; Garrigó, Montserrat; González-Martin, Julià; Greub, Gilbert; Hrabak, Jaroslav; Ingebretsen, André; Mediavilla-Gradolph, Maria Concepción; Oviaño, Marina; Palop, Begoña; Pranada, Arthur B; Quiroga, Lidia; Ruiz-Serrano, Maria Jesús; Rodríguez-Sánchez, Belén

    Scientific reports, 01/2022, Letnik: 12, Številka: 1
    Journal Article

    The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.