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Arnold, Mitchell T; Temte, Jonathan L; Barlow, Shari K; Bell, Cristalyne J; Goss, Maureen D; Temte, Emily G; Checovich, Mary M; Reisdorf, Erik; Scott, Samantha; Guenther, Kyley; Wedig, Mary; Shult, Peter; Uzicanin, Amra
PloS one, 10/2020, Letnik: 15, Številka: 10Journal Article
We analyzed 4,352 participant- and staff-collected respiratory specimens from 2,796 subjects in the Oregon Child Absenteeism due to Respiratory Disease Study. Trained staff collected oropharyngeal specimens from school-aged children with acute respiratory illness while household participants of all ages collected their own midturbinate nasal specimens in year one and anterior nasal specimens in year two. Human ribonuclease P levels were measured using RT-PCR for all staff- and participant-collected specimens to determine adequacy, defined as Cycle threshold less than 38. Overall, staff- and participant-collected specimens were 99.9% and 96.4% adequate, respectively. Participant-collected midturbinate specimens were 95.2% adequate in year one, increasing to 97.2% in year two with anterior nasal collection. The mean human ribonuclease P Cycle threshold for participant-collected specimens was 31.18 in year one and 28.48 in year two. The results from this study suggest that community-based participant collection of respiratory specimens is comparable to staff-collected oropharyngeal specimens, is feasible, and may be optimal with anterior nasal collection.
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in: SICRIS
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