Investigations are being conducted on chitosan-based polymeric films that have been encapsulated with lanthanum, samarium, and graphene oxide. The primary distinguishing signals of chitosan (CS) and other components were seen in the FTIR spectra. Additionally, the interaction of the CS with the oxy-components clearly increased the crystallinity, as shown by the XRD pattern. The optical data shows that both Sm2O3 and GO cause diminishing in band gap while lanthanum oxide cause a significant widening in it. Additionally, this study discusses the impact of mixed oxides insertion on the cytotoxicity of La2O3/Sm2O3/GO@CS film toward human lung cell-line. Cell viability is seen to be 123.21 % when 2.2 g/ml is utilized, and 117.95 % when 4.4 g/ml is used. Viability percentages of 29.49 and 27.31 % are obtained using 2250 and 4500 g/ml, respectively. The obtained data showed that the CS-based films behaved in a promising way when it came to healing wounds. Display omitted •La2O3/Sm2O3/GO@CS scaffold is fabricated via casting technique.•Sm2O3 and GO cause diminishing in band gap while lanthanum oxide causes a significant widening in it.•Cytotoxicity of La2O3/Sm2O3/GO@CS film toward human lung cell-line.•Cell viability is seen to be 123.21 % when 2.2 g/ml is utilized, and 117.95 % when 4.4 g/ml is used.