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González-Durán, Elizabeth; Contreras-Pérez, Cudberto U.; Caceres, Diego H.; Ríos-Rosas, Claudia; Piñón-Ortega, Javier de J.; Téllez-Saucedo, Ma. Dolores; Marín-Suro, Edgar S.; Wong-Arámbula, Claudia E.; Moreno-Escobar, E. Alejandra; Ramírez-González, José Ernesto; Ramírez-Barrios, J. Gabriela; Montes-Colima, Norma A.; Lockhart, Shawn R.; Martínez-Montiel, Nancy; Martínez-Contreras, Rebeca D.; García-Ruíz, Pablo; Salazar-Sánchez, Ma. Isabel; Hernández-Rivas, Lucia; López-Martínez, Irma
Archives of microbiology, 09/2022, Letnik: 204, Številka: 9Journal Article
Identification of the emerging multidrug-resistant yeast Candida auris is challenging. Here, we describe the role of the Mexico national reference laboratory Instituto de Diagnóstico y Referencia Epidemiológicos Dr. Manuel Martínez Báez (InDRE) and the Mexican national laboratory network in the identification of C. auris . Reference identification of six suspected isolates was done based on phenotypic and molecular laboratory methods, including growth in special media, evaluation of isolate micromorphology, and species-specific PCR and pan-fungal PCR and sequencing. The four C. auris isolates identified were able to grow on modified Sabouraud agar with 10% NaCl incubated at 42 °C. With one exception, isolates of C. auris were spherical to ovoid yeast-like cells and blastoconidia, with no hyphae or pseudohyphae on cornmeal agar. C. auris isolates were resistant to fluconazole. Species-specific and pan-fungal PCR confirmed isolates as C. auris . Sequence analysis revealed the presence of two different C. auris clades in Mexico, clade I (South Asia) and clade IV (South America).
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
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Vir: Osebne bibliografije
in: SICRIS
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