•The cause of fatigue failure in steel wire rope suspenders on suspension bridges has been identified.•The recommended approach of utilizing auxiliary cables is successful in decreasing tensile-bending fatigue stress levels.•These discoveries are significant for guiding the development and upkeep of comparable infrastructure ventures. In consideration of the significant impact of train loads on the fatigue of suspenders in railway bridges, this paper focuses on the first railway suspension bridge in China − the Jinsha River Railway Suspension Bridge. The study analyzes the force characteristics and bending failure features of the steel wire ropes in railway suspension bridges, aiming to provide insights into the design of such structures. Firstly, a finite element model of the Jinsha River Railway Suspension Bridge is established in this paper. The natural frequencies are analyzed and compared with on-site measurements to validate the accuracy of the finite element model. Secondly, the tension force amplitude and angular displacement amplitude of the suspenders are analyzed under design loads, and the failure characteristics of the cables are experimentally examined. Subsequently, a wear fatigue test for steel wire rope is designed, evaluating the fatigue life of the suspender in the bridge. The computational results are compared with experimental data to validate their accuracy. Finally, structural measures to reduce the bending fatigue of railway suspension bridge suspenders are proposed to ensure their safety during the service life. The study reveals that the presence of bending stress exacerbates the fatigue failure of suspension cables in railway suspension bridges, with the failure mode identified as fatigue failure due to the tension-bending-wear coupling effect. Furthermore, our findings indicate that the implementation of auxiliary cables can reduce the displacement differential at both ends of the suspension cables by approximately 4/5, and decrease the axial tensile stress amplitude by about 3/4. Consequently, this significantly enhances the fatigue life of the suspension cables.