E-viri
Recenzirano
Odprti dostop
-
Guo, Yuting; Li, Di; Zhang, Siwei; Yang, Yanrui; Liu, Jia-Jia; Wang, Xinyu; Liu, Chong; Milkie, Daniel E.; Moore, Regan P.; Tulu, U. Serdar; Kiehart, Daniel P.; Hu, Junjie; Lippincott-Schwartz, Jennifer; Betzig, Eric; Li, Dong
Cell, 11/2018, Letnik: 175, Številka: 5Journal Article
In eukaryotic cells, organelles and the cytoskeleton undergo highly dynamic yet organized interactions capable of orchestrating complex cellular functions. Visualizing these interactions requires noninvasive, long-duration imaging of the intracellular environment at high spatiotemporal resolution and low background. To achieve these normally opposing goals, we developed grazing incidence structured illumination microscopy (GI-SIM) that is capable of imaging dynamic events near the basal cell cortex at 97-nm resolution and 266 frames/s over thousands of time points. We employed multi-color GI-SIM to characterize the fast dynamic interactions of diverse organelles and the cytoskeleton, shedding new light on the complex behaviors of these structures. Precise measurements of microtubule growth or shrinkage events helped distinguish among models of microtubule dynamic instability. Analysis of endoplasmic reticulum (ER) interactions with other organelles or microtubules uncovered new ER remodeling mechanisms, such as hitchhiking of the ER on motile organelles. Finally, ER-mitochondria contact sites were found to promote both mitochondrial fission and fusion. Display omitted •Super-resolution live-cell imaging up to 266 fps at 97-nm resolution•Hitchhiking interactions among organelles remodel ER and mitochondrial networks•ER-mitochondrion contacts promote coalescence of mitochondrial membranes•Collision of late endosomes or lysosomes carried along microtubules split ER tubules A new approach for visualizing dynamic processes within cells offers insight into membrane-membrane contact interactions and microtubule function.
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|
Vir: Osebne bibliografije
in: SICRIS
To gradivo vam je dostopno v celotnem besedilu. Če kljub temu želite naročiti gradivo, kliknite gumb Nadaljuj.