The aim of this work was to evaluate the incorporation of a freeze-dried probiotic strain ( Lactobacillus plantarum CIDCA 83114) into zeolites. The bacteria-zeolite mixture was added to poultry feed together with thyme, and the obtained product was stored for 60 days at 25 °C and 60–70% relative humidity. The ability of the obtained product to remove aflatoxin B1 (AFB1) was studied. The highest bacterial viability was observed when 50% w/w bacteria were added to zeolites. The bacterial:zeolite mixtures were then incorporated into poultry feed containing or not thyme. Initial counts of L. plantarum were in the range of 1–2 × 10 8 CFU/g for all samples. In all cases, bacterial viability decreased one logarithmic order after 20 days of storage, and three logarithmic orders after 60 days. No significant viability loss was observed after exposure of the poultry feed to gastro-intestinal conditions. Freeze-dried L. plantarum and zeolite were able to remove AFB1, with an average reduction of 20 and 80%, respectively. Moreover, the freeze-dried bacteria-zeolite mixture was capable to remove up to 90% AFB1. This work contributes to enhance the nutritional quality of poultry feed, with a strong impact in production.