Trait mindfulness appears to be related to lower levels of negative affective symptoms, but it remains uncertain which facets of mindfulness are most important in this relationship. Accordingly, the present meta-analysis examined studies reporting correlations between affective symptoms and trait mindfulness as assessed by the Five Facet Mindfulness Questionnaire. A comprehensive search yielded 148 eligible studies, comprising 157 distinct samples and 44,075 participants. The weighted mean correlation for affective symptoms and overall trait mindfulness was r = −0.53. Among mindfulness facets, Nonjudge (r = −0.48) and Act with Awareness (r = −0.47) demonstrated the largest correlations, followed by Nonreact (r = −0.33) and Describe (r = −0.29). Observe was not significantly correlated with affective symptoms. No significant differences in the strength of correlations were found between anxiety, depression and posttraumatic stress disorder (PTSD) symptoms, though symptoms of generalized anxiety disorder exhibited a weaker negative relationship with the Describe facet compared to PTSD symptoms. Describe also showed a stronger relationship with affective symptoms in Eastern samples compared to Western samples, whereas Western samples had a stronger relationship with Nonjudge. These results provide insight into the nature of the association between trait mindfulness and negative affect. •Higher trait mindfulness is associated with fewer negative affective symptoms.•Relationships with symptoms vary across different mindfulness facets.•Nonjudging and Acting with Awareness show the strongest correlations.•Observing is not meaningfully correlated with symptoms in non-meditators.•Relationships were mostly consistent across symptom type (e.g. anxiety, depression).