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Sánchez-Martínez, José David; Alvarez-Rivera, Gerardo; Gallego, Rocio; Fagundes, Mariane Bittencourt; Valdés, Alberto; Mendiola, Jose A.; Ibañez, Elena; Cifuentes, Alejandro
Food Chemistry: X, 03/2022, Letnik: 13Journal Article
•Orange juice by-products valorized by green PLE process.•Terpenoid-rich PLE extract with enhanced in-vitro neuroprotective activity.•Molecular docking between terpenoids and active sites of the target enzymes.•Selected PLE extracts show human cell-based neuroprotection capacity. Pressurized liquid extraction (PLE) conditions were optimized to improve the recovery of orange (Citrus sinensis) by-products terpenoids. The neuroprotective potential of the PLE extracts were tested against a set of in-vitro assay (antioxidant (ABTS), reactive oxygen/nitrogen species (ROS/RNS)) as well as enzymatic tests (acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and lipoxygenase (LOX)). Gas chromatography coupled to high-resolution mass spectrometry (GC-q-TOF-MS) analysis revealed a higher enrichment in mono- and sesquiterpenoids of the PLE extracts with the highest neuroprotection capacity. In-silico molecular docking analysis showed the specific interaction of representative terpenoids with enzymes active sites. The results demonstrate that the selected extract at 100 °C and 30 minutes possesses high antioxidant (ABTSIC50 = 13.5 μg mL−1; ROSIC50 = 4.4 μg mL−1), anti-cholinesterase (AChEIC50 = 137.1 vg L−1; BChEIC50 = 147.0 μg mL−1) and anti-inflammatory properties (against IL-6 and LOXIC50 = 76.1 μg mL−1), with low cytotoxicity and protection against L-glutamic acid in cell models.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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