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Davies, Stephen R.; Jones, Kai; Goldys, Anna; Alamgir, Mahuiddin; Chan, Benjamin K. H.; Elgindy, Cecile; Mitchell, Peter S. R.; Tarrant, Gregory J.; Krishnaswami, Maya R.; Luo, Yawen; Moawad, Michael; Lawes, Douglas; Hook, James M.
Analytical and bioanalytical chemistry, 04/2015, Letnik: 407, Številka: 11Journal Article
Quantitative NMR spectroscopy (qNMR) has been examined for purity assessment using a range of organic calibration standards of varying structural complexities, certified using the traditional mass balance approach. Demonstrated equivalence between the two independent purity values confirmed the accuracy of qNMR and highlighted the benefit of using both methods in tandem to minimise the potential for hidden bias, thereby conferring greater confidence in the overall purity assessment. A comprehensive approach to purity assessment is detailed, utilising, where appropriate, multiple peaks in the qNMR spectrum, chosen on the basis of scientific reason and statistical analysis. Two examples are presented in which differences between the purity assignment by qNMR and mass balance are addressed in different ways depending on the requirement of the end user, affording fit-for-purpose calibration standards in a cost-effective manner.
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JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
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in: SICRIS
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