Up to 58% of NHsub.3 emissions can be reduced through the use of the urease inhibitor Atmowellsup.® in cattle farming. Automated manure scraper and drag hose systems should be used to apply urease inhibitors (UIs) to stable floors. Proof of safe use is also needed. A risk assessment of the urease inhibitor PPDA was conducted utilising estimated and calculated exposure data along with established and verified model calculation tools. Risk assessment models can be improved using measured dermal exposure data. The fluorescent dye pyranine and a Tyveksup.® collector were used to measure exposure. Tyveksup.® was attached to a lifelike cow dummy. Regarding the drag hose system, two worst-case scenarios were considered: over the cow and passing the cow. Regarding the robot application system, a 0 m frontal scenario was considered. The over the cow scenario exhibited the highest dermal exposure, with 1.5 mg of PPDA/cow per application run. The robot scenario exhibited the smallest amount, with 0.8 mg of PPDA/cow per application run. The risk of using PPDA was already excluded by model calculation tools in a risk assessment. Compared with the estimated and calculated dermal exposure values, the measured dermal exposure levels were reduced by a factor of two to six.