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Zielonka, Jacek; Lambeth, J. David; Kalyanaraman, Balaraman
Free radical biology & medicine, 12/2013, Letnik: 65Journal Article
L-012, a luminol-based chemiluminescent (CL) probe, is widely used in vitro and in vivo to detect NADPH oxidase (Nox)-derived superoxide (O₂ ⁻) and identify Nox inhibitors. Yet understanding of the free radical chemistry of the L-012 probe is still lacking. We report that peroxidase and H₂O₂ induce superoxide dismutase (SOD)-sensitive, L-012-derived CL in the presence of oxygen. O₂ ⁻ alone does not react with L-012 to emit luminescence. Self-generated O₂ ⁻ during oxidation of L-012 and luminol analogs artifactually induce CL inhibitable by SOD. These aspects make assays based on luminol analogs less than ideal for specific detection and identification of O₂ ⁻ and NOX inhibitors.
