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Anderson, Kayla Nicole; Moaven, Shiva; Cozzolino, Anthony; D'Auria, John C.
The FASEB journal, April 2018, 2018-04-00, Letnik: 32, Številka: S1Journal Article
Polyamines are abundant in nature, and the methylation of polyamines is a common enzymatic step in plants, especially plants containing alkaloids. This includes the family of compounds called granatane alkaloids. Pomegranate (Punica granatum) is one of the most commonly known granatane alkaloid‐producing plants. Pomegranate extracts are widely used for their antioxidant, anti‐inflammatory, and anticarcinogenic effects. 1 One of the proposed enzymes in the hypothetical pathway for the formation of granatane alkaloids is cadaverine N‐methyl transferase.2 It is important to find a method of synthesis for N‐methyl cadaverine (N‐methylpentane‐1,5‐diamine) and other N‐methyl polyamines, because they can be utilized as a standard for quantification purposes as well as providing a positive control when evaluating enzymatic activities of putative alkaloid producing N‐methyl transferases. The synthesis of N‐methyl cadaverine was employed by utilizing a substitution reaction between N‐methylbenzylamine and 5‐bromo‐valeronitrile, removing the protecting group (or benzyl ring) attached to the methylated amine, and reduction of the nitrile. 3 This is from the Experimental Biology 2018 Meeting. There is no full text article associated with this published in The FASEB Journal.
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