碩士 國立中興大學 生物化學研究所 87 Abstract The cleavage of DNA by vanadium(V)-preoxo complex under photo-irradiation conditions at neutral pH has been demonstrated (e.g., Inorg. Chem. 1997,36,1276-1277). In this study, we report the binding interaction between a vanadium(V)-peroxo complex, VO(O2)2(5-NH2phen) (where 5-NH2phen = 5-amino-1,10-phenanthroline), to seven synthetic oligo-nucleotides, 5’-GC-GATACC-GC---(I), 5’-GC-GGTATC-GC---(II), 5’-d(A)15---(IV) 5’-d(T)10---(V), 5’-AA-CCCCCC-TT---(VI) 5’-TT-GGGGGG-AA---(VII) and their mixture I+II、VI+VII, as well as a self-complementary oligermer 5’-GC-GATATC-GC---(III). The sequence-directed binding of oligo-DNA to vanadium(V)-peroxo complex was established. The fluorescence emission spectroscopy was used for monitoring the complex formation between vanadium(V)-preoxo complexes and DNA oligermers. The substrate III contains the recognition sequence 5’-GATATC- but not in substrates I, II, IV, V, VI, VII. In this study, the fluorescence emission of this complex was found to be