SrCe 0.9Y 0.1O 3− δ (SCY10) powder with a pure perovskite phase is prepared by solid-state reaction method. NiO is dispersed uniformly in SCY10 powder to fabricate NiO-SCY10 anode substrate. The starting powder, the mixture of SrCO 3, CeO 2 and Y 2O 3, is deposited directly on the green substrate instead of SCY10 powder by spin coating. After co-firing at 1300 °C for 3 h, the starting powder reacts to form SCY10 top layer on the substrate. SEM micrographs show that the top layer is defect-free and adheres well with the anode substrate without any delamination. A single fuel cell is assembled with anode-supported SCY10 membrane as electrolyte membrane and Ag as cathode. The electrochemical property of the fuel cell is tested with hydrogen as fuel in the temperature range of 600–800 °C. The open circuit voltage (OCV) reaches 1.05 V at 800 °C, and the maximum power density is 50 mW cm −2, 155 mW cm −2, 200 mW cm −2 at 600 °C, 700 °C, 800 °C, respectively.