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Persistence of viruses in upper respiratory tract of children with asthmaMarin, Jožica ...Objectives: Nasopharyngeal swabs of 50 asthmatic children in the symptom-free period were examined for the presence of adenoviruses, rhinoviruses and coronaviruses. A control group of 20 healthy ... individuals was included in this study. Methods: A polymerase chain reaction was used to detect adenovirus DNA and rhinovirus and coronavirus complementary DNA. The fragments of amplified genetic material were visualized with the use of agarose gel electrophoresis. Results: Adenovirus DNA was found in 78.4% of asthmatic children, rhinovirus RNA in 32.4% and coronavirus RNA in 2.7%. Adenovirus DNA was detected in one of the 20 nasopharyngeal swabs of healthy controls; the rest of the control samples were negative. Conclusions: The persistent presence of viruses in the upper respiratory tract of asthmatic children shows a possible connection between viral infections and asthma.Source: The Journal of infection. - ISSN 0163-4453 (Letn. 41, št. 1, 2000, str. 69-72)Type of material - article, component partPublish date - 2000Language - englishCOBISS.SI-ID - 14902233
Author
Marin, Jožica |
Jeler-Kačar, Dora |
Levstek, V... |
Maček, Vasilija
Topics
Respiratory tract infections |
Complications |
Virology |
Nasopharyngeal diseases |
Complications |
Virology |
Asthma |
Complications |
Virology |
Child |
Adult |
Rhinovirus |
Isolation and purification |
Genetics |
Polymerase chain reaction |
Picornaviridae infections |
Complications |
Virology |
Nasopharynx |
Virology |
Adenoviridae |
Genetics |
Isolation and purification |
Adenoviridae infections |
Complications |
Virology |
Coronaviridae infections |
Complications |
Virology |
Coronavirus |
Isolation and purification |
Genetics |
DNA primers |
DNA, viral |
Chemistry |
Isolation and purification |
Electrophoresis, agar gel |
Astma |
Dihala, infekcije |
Nazofaringealne bolezni |
Adenoviridae |
Adenoviridae infekcije |
Coronaviridae, infekcije |
Coronavirus |
DNA virusna |
DNA začetki |
Elektroforeza, agar gel |
Nazofarinks |
Odrasli |
Otrok |
Picornaviridae, infekcije |
Polimeraza, verižna reakcija |
Rinovirus
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Database name | Field | Year |
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Marin, Jožica | 03172 |
Jeler-Kačar, Dora | 16123 |
Maček, Vasilija | 05323 |
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