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Razgradnja ksilana z različnimi ksilanazami vampne bakterije Pseudobutyrivibrio xylanivorans Mz5T = Xylan degradation by different xylanases of rumen bacterium Pseudobutyrivibrioxylanivorans Mz5TČepeljnik, Tadej ; Fidler, Katarina ; Marinšek-Logar, Romana, 1960-Uporaba ksilanaz kot krmnih dodatkov je pri monogastričnih živalih zelo obetavna, saj so njihov ugoden učinek na zdravstveno stanje in prirast živali potrdile številne raziskave. Pred kratkim je bila ... opisana nova vrsta vampne bakterije P. xylanivorans s tipskim sevom Mz5T, ki je močno ksilanolitična. Nekaj njenih ksilanolitičnih encimov je že opisanih, preiskati pa želimo se ostale, da bi ugotovili vzrok visoke ksilanolitične aktivnosti in možnosti uporabe bakterije kot probiotika pri monogastričnih živalih (prašiči, perutnina) ali encimov kot krmnih dodatkov. Iz celičnega izvlečka smo uspeli delno očistiti ksilanazi, veliki 44 kDa in 81 kDa in delno proučiti njuno encimsko delovanje. Primerjali smo tudi encimsko delovanje na celično površino vezanih ksilanaz s ksilanazami celičnega izvlečka. 44 kDa ksilanaza je eksoksilanaza brez ß-ksilozidazne aktivnosti, pri ksilanazi velikosti 81 kDa predvidevamo, da gre za endoksilanazo. Ksilanolitični encimi celične površine učinkovito razgrajujejo ksilan, vendar niso sposobni cepiti ksilobioze. Le-to razgradijo ksilanolitični encimi v notranjosti celice.Source: Acta agriculturae Slovenica. - ISSN 1581-9175 (Letn. 84, št. 2, 2004, str. 141-151)Type of material - article, component partPublish date - 2004Language - slovenianCOBISS.SI-ID - 1676936
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Database name | Field | Year |
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Čepeljnik, Tadej | 21437 |
Fidler, Katarina | 30615 |
Marinšek-Logar, Romana, 1960- | 08404 |
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