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Optimizacija analiznega postopka merjenja aktivnosti lipoproteinske lipaze v rakavem tkivu = Optimization of an analytical procedure of lipoprotein lipase activity measurement in cancer tissueBilač-Krašnja, Mojca ; Černe, DarkoRaziskave kažejo na povezavo med aktivnostjo lipoproteinske lipaze (LPL; EC 3.1.1.34) in rastjo rakavega tkiva. Zaradi odsotnosti enostavnih metod za merjenje njene aktivnosti je intenzivnejše ... proučevanje vloge LPL v razvoju raka omejeno. V raziskavi smo želeli ugotoviti, ali je postopek temelječe na izluževanju LPL iz tkiva z raztopino heparina in albumina ter merjenju aktivnosti encima v eluatu s komercialnim postopkom LPL Activity Kit (Roar Biomedical, New York, ZDA) primeren za merjenje aktivnosti LPL v rakavem tkivu. Ugotovili smo, da je postopek zanesljiv le ob upoštevanju dodatnih, v naši raziskavi ugotovljenih priporooeil glede stabilnosti izluževalne raztopine in substratne emulzije, merjenja v encimski reakciji nastalega fluorescenoenega produkta vsakih pet minut, individualne ocene ustreznosti poteka encimske reakcije in standardizacije aktivnosti encima na maso tkiva (nkat/kg). Z vpeljanim postopkom smo ugotovili, da je aktivnost LPL v rakavem pljučnem tkivu v povprečju 1,94-krat višja kot v sosednjem, navidezno neprizadetem pljuoenem tkivu istega bolnika (p < 0,001; 10 bolnikov z nedrobnoceliočnim pljučnim rakom brez metastaz). Zaključujemo lahko, da je vpeljan postopek primeren za merjenje aktivnosti LPL v tkivih in za nadaljnje proučevanje vloge encima pri preskrbi rakavega tkiva z maščobami, nujnimi zarast tkiva.Source: Farmacevtski vestnik : strokovno glasilo slovenske farmacije = pharmaceutical journal of Slovenia. - ISSN 0014-8229 (Letn. 60, št. 1, 2009, str. 21-26)Type of material - article, component partPublish date - 2009Language - slovenianCOBISS.SI-ID - 2540401
Author
Bilač-Krašnja, Mojca |
Černe, Darko
Topics
lipoproteinska lipaza |
rakavo tkivo |
encimska sposobnost
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source: Farmacevtski vestnik : strokovno glasilo slovenske farmacije = pharmaceutical journal of Slovenia. - ISSN 0014-8229 (Letn. 60, št. 1, 2009, str. 21-26)
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Bilač-Krašnja, Mojca | ![]() |
Černe, Darko | 15099 |
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