-
Proinflammatory effects of bare and PEGylated ORMOSIL-, PLGA- and SUV-NPs on monocytes and PMNs and their modulation by f-MLPSegat, Daniela ...We wanted to test the proinflammatory effects of vinyltriethoxysilane-based organically modified silica nanoparticles (ORMOSIL-NPs) in vitro on blood leukocytes. Materials & methods: Cell ... selectivity, cytokines/chemokines and O2- production were analyzed using nonpolyethylene glycol (PEG)ylated and PEGylated ORMOSIL-NPs, poly(lactic-co-glycolic acid) (PLGA)-NPs and small unilamellar vesicles (SUV)-NPs. Results: ORMOSIL-NPs mostly bound to monocytes while other NPs to all leukocyte types similarly. Cell capture of PEGylated-NPs decreased strongly (ORMOSIL), moderately (PLGA) and weakly (SUV). Bare ORMOSIL-NPs effectively stimulated the production of IL-1b/IL-6/TNF-a/IL-8 by monocytes and of IL-8 by polymorphonuclear leukocytes(PMNs). NP PEGylation inhibited such effects only partially. Formyl-methionine-leucine phenylalanine (f-MLP) further increased the release of cytokines/chemokines by monocytes/PMNs primed with bare and PEGylated ORMOSIL-NPs. PEGylated SUV-NPs, bare and PEGylated ORMOSIL- and PLGA-NPs sensitize PMNs and monocytes to secrete O2- upon f-MLP stimulation. Conclusion: ORMOSIL-NPs are preferentially captured by circulating monocytes but stimulate both monocytes and PMNs per se or by sensitizing them to another agonist (f-MLP). PEG-coating confers stealth effects but does not completely eliminate leukocyte activation. Safe nanomedical applications require the evaluation of both intrinsic and cooperative proinflammatory potential of NPs.Source: Nanomedicine. - ISSN 1743-5889 (Vol. 6, no. 6, 2011, str. 1027-1046)Type of material - article, component partPublish date - 2011Language - englishCOBISS.SI-ID - 3033713
Author
Segat, Daniela |
Tavano, Regina |
Donini, Marta |
Rio-Echevarria, Iria |
Rojnik, Matija |
Kocbek, Petra, farmacevtka |
Kos, Janko, 1959- |
Iratni, Selma |
Schegelmann, Dietrich |
Mancin, Fabrizio |
Dusi, Stefano |
Papini, Emanuele
Topics
citokini |
ORMOSIL nanodelci |
PLGA nanodelci |
levkociti |
monociti |
diagnostika
Shelf entry
Permalink
- URL:
Impact factor
Access to the JCR database is permitted only to users from Slovenia. Your current IP address is not on the list of IP addresses with access permission, and authentication with the relevant AAI accout is required.
Year | Impact factor | Edition | Category | Classification | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Select the library membership card:
DRS, in which the journal is indexed
Database name | Field | Year |
---|
Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
---|---|
Segat, Daniela | |
Tavano, Regina | |
Donini, Marta | |
Rio-Echevarria, Iria | |
Rojnik, Matija | 31397 |
Kocbek, Petra, farmacevtka | 24402 |
Kos, Janko, 1959- | 04648 |
Iratni, Selma | |
Schegelmann, Dietrich | |
Mancin, Fabrizio | |
Dusi, Stefano | |
Papini, Emanuele |
Select pickup location:
Material pickup by post
Notification
Subject headings in COBISS General List of Subject Headings
Select pickup location
Pickup location | Material status | Reservation |
---|
Please wait a moment.