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Enhancing effect of 100.414-kHz electromagnetic field produced by Defender's pulse generator on the ChIFN [gamma]-like molecule inducing capacity of Lens culinaris agglutinin and 10% PBS washouts of different holocene minerals [Elektronski vir]Filipič, Bratko, 1950- ...Macrophages play key role in host defense and tissue repair, and thus understanding regulation of their function is important. For instance, our previous results have shown that in chicken macrophage ... system (CoMA cell line), application of a pulse of electromagnetic fields of frequencies 0.618, 1.054, 5.229, and 100.414 kHz induces production of interferon [gamma]-like molecules. In this study, we have shown that the electromagnetic field of 100.414 kHz is themost effective in inducing synthesis of chicken interferon [gamma] and chicken interferon [gamma]-like molecules in CoMA cells, especially when combined with Lens culinaris agglutinin and 10% phosphate-buffered saline washouts of different Holocene minerals. A2-minute pulse of electromagnetic fieldwas produced byDefender's pulse generator. Both chicken interferon [gamma] and chicken interferon [gamma]-like molecules from the cell supernatant were evaluated by an antiviral assay and were also analyzedwith reverse-phase high-performance liquid chromatography on Phenomenex, Aeris peptide columns. Our results show that application of a single inducing factor (Lens culinaris agglutinin, 100.414 kHz electromagnetic field, 10% phosphate buffer saline washout) or combined usage of 2 of them moderately stimulated production of chicken interferon [gamma]-like molecules (from 1.550 to 48.028 IU/mL), whereas the combination of 10% phosphate-buffered saline washout of Koprivnica rock + Lens culinaris agglutinin + 100.414 kHz/9 V resulted in an output of 162.122 IU/mL. Hence, we may conclude that a combined use of electromagnetic field, Holocene minerals, and Lens culinaris agglutinin greatly stimulates synthesis of chicken interferon [gamma]-like molecules in CoMA cells.Source: Technology in cancer research and treatment [Elektronski vir]. - ISSN 1533-0338 (Vol. 18, 2019, str. 1-8)Type of material - e-articlePublish date - 2019Language - englishCOBISS.SI-ID - 34177497
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Filipič, Bratko, 1950- | 05320 |
Rihar, Klemen | |
Exel Gregorič, Dunja | |
Gradišnik, Lidija, 1972- | 20420 |
Pereyra Gonzales, Adriana | |
Đermić, Damir | |
Daničić, Časlav | |
Mazija, Hrvoje |
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