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Just Seeing Is Not Enough for Believing [Elektronski vir] : immunolabelling as indisputable proof of SARS-CoV-2 virions in infected tissueErman, Andreja ...Background: There is increasing evidence that identification of SARS-CoV-2 virions by transmission electron microscopy could be misleading due to the similar morphology of virions and ubiquitous cell ... structures. This study thus aimed to establish methods for indisputable proof of the presence of SARS-CoV-2 virions in the observed tissue. Methods: We developed a variant of the correlative microscopy approach for SARS-CoV-2 protein identification using immunohistochemical labelling of SARS-CoV-2 proteins on light and electron microscopy levels. We also performed immunogold labelling of SARS-CoV-2 virions. Results: Immunohistochemistry (IHC) of SARS-CoV-2 nucleocapsid proteins and subsequent correlative microscopy undoubtedly proved the presence of SARS-CoV-2 virions in the analysed human nasopharyngeal tissue. The presence of SARS-CoV-2 virions was also confirmed by immunogold labelling for the first time. Conclusions: Immunoelectron microscopy is the most reliable method for distinguishing intracellular viral particles from normal cell structures of similar morphology and size as virions. Furthermore, we developed a variant of correlative microscopy that allows pathologists to check the results of IHC performed first on routinely used paraffin-embedded samples, followed by semithin, and finally by ultrathin sections. Both methodological approaches indisputably proved the presence of SARS-CoV-2 virions in cells.Source: Viruses [Elektronski vir]. - ISSN 1999-4915 (Vol. 13, iss. 9, 2021, str. 1-9)Type of material - e-article ; adult, seriousPublish date - 2021Language - englishCOBISS.SI-ID - 76924931
Author
Erman, Andreja |
Wechtersbach, Karmen |
Velkavrh, Dane |
Pleško, Jerica |
Frelih, Maja |
Kojc, Nika
Topics
SARS-CoV-2 |
immunolabelling |
microscopy |
SARS-CoV-2 |
imuno označevanje |
mikroskopija
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Erman, Andreja | 23916 |
Wechtersbach, Karmen | 50899 |
Velkavrh, Dane | 18362 |
Pleško, Jerica | 36130 |
Frelih, Maja | 53997 |
Kojc, Nika | 22462 |
Source: Personal bibliographies
and: SICRIS
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