BF, Dept. of Food Science and Technology, Lj. (BFZIV)
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13.
Ultrastruktura celic krompirjevih rastlin okuženih z virusom PVY[zgoraj]NTN po zamrzovalnih postopkih v elektronski mikroskopiji : diplomska naloga = Ultrastructure of potato plant cells infected with potato virus Y[up]NTN after cryofixation in electron microscopy : graduation thesis
Rukavina, Matej
Primerjali smo najnovejǴe tehnike yamryovanja s klasiřnimi kemiřnimi postopki priprave bioloǴkih vyorcev ya opayovanje v presevnem elektronskem mikroskopu. V poskusih smo uporabili vrǴiřke, liste, ...stebla in korenine krompirja Solanum tuberosum sorte Dšsirše vygojene s tkivno kulturo, sistemsko okuÍene y virusom PVZNTN. Uporabili smo metodo yamryovanja v propanuin yamryovanja pod visokim pritiskom, katerima sledi kriosubstitucija. Reyultate smo primerjali y reyultati kemiřno fiksiranih tkiv. Kemiřno smo fiksirali s kombinacijo glutaraldehida in formaldehida ter y oymijevim tetraoksidom. Primerjali smo tudi vpliv epoksidnih in metakrilatnih elektronsko mikroskopskih smol (ERL in HM20) na ultrastrukturo ydravih rastlin. Raylik v ultrastrukturi med ydravimi in s PVZNTN okuÍenimi rastlinaminismo opayili. Prav tako nismo opayili raylik v ultrastrukturi med tkivi vloÍenimi v smolo ERL in HM20. Ultrastrukturo rastlinskih celic smo bolje ohranili y yamryovalnimi tehnikami kot s kemiřnimi. Na osnovi reyultatovmenimo, da sta yamryovanje pod visokim pritiskom in yamryovanje v propanu primernejǴi metodi priprave rastlinskega vyorca ya opayovanje ultrastrukture rastlinskih celic, od kemiřne fiksacije.
Type of material - undergraduate thesis
; adult, serious
Publication and manufacture - Ljubljana : [M.Rukavina] ; [BF, Enota medoddelčnega študija mikrobiologije], 1999
Študij mikrobiologije DN 0000000042 IN: 0011856
Študij mikrobiologije DN 42 IN: 0011856
available - outside loan, loan period: 1 months
Študij mikrobiologije DN 0000000042 a IN: 0011857
Študij mikrobiologije DN 42 a IN: 0011857
available - outside loan, loan period: 1 months
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