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Influence of some environmental factors on Rhizopus nigricans submerged growth in the form of pelletsŽnidaršič Plazl, Polona ; Komel, Radovan ; Pavko, AleksanderMorphological changes of a steroid transforming filamentous fungus Rhizopus nigricans were studied by altering submerged growth conditions at inoculum sizes previously determined to favor pelleted ... growth. Beside the main classification between pelleted and clumpy growth forms, the size, concentration and structure of pellets were characterized at different cultivating temperature, initial pH value, medium composition, additives, and aeration conditions. Initial pH below 4 and above 7, the presence of ▫$Ca^{2+}$▫ andTween-80 gave rise to the clumpy growth, otherwise pelleted growth prevailed. Among tested factors the pellet size was mainly influenced by the medium. Theformation of smooth pellets, prerequisite for further application in the process of steroid biotransformation, resulted in cultivations at lower temperature, high agitation rates in shaken cultures without baffles and at high nitrogen concentration.Source: World journal of microbiology & biotechnology. - ISSN 0959-3993 (Vol. 16, no. 7, 2000, str. 589-593)Type of material - article, component partPublish date - 2000Language - englishCOBISS.SI-ID - 23284485
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source: World journal of microbiology & biotechnology. - ISSN 0959-3993 (Vol. 16, no. 7, 2000, str. 589-593)
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Database name | Field | Year |
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Žnidaršič Plazl, Polona | 11250 |
Komel, Radovan | 06135 |
Pavko, Aleksander | 06707 |
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