Faculty of Pharmacy, Lj. (FFALJ)
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Izražanje gena za (pro)katepsin X v celični liniji PC-12 ob prisotnosti amiloida beta = Expression of (pro)cathepsin X gene in the PC-12 cell line in the presence of amyloid beta : magistrski program laboratorijska biomedicinaŠketelj, BlažV okviru magistrske naloge smo želeli preveriti ali citotoksični fragment A[beta](25-35) vpliva na izražanje gena za (pro)katepsin X v celični liniji PC-12. Celična linija PC-12 predstavlja dober ... model za preučevanje nevrodegeneracije, saj izraža tudi katepsin X v neaktivni obliki prokatepsina X, ki se nahaja v lizosomih. Nediferencirane celice PC-12 smo inkubirali z A[beta](25-35) v osmih časovnih intervalih (po 3 ure) in v lizatih celic s testom ELISA izmerili količino (pro)katepsina X ter s kinetično metodo encimske aktivnosti s fluorogenim substratom izmerili množino aktivnega katepsina X. Rezultate smo normirali na količino celokupnih proteinov, ki smo jih kvantificirali z metodo po Lowry-ju. Ugotovili smo, da se količina celokupnega katepsina X (pro in aktivna oblika) statistično značilno poveča že v 3 urah inkubacije z A[beta](25-35) in ostane povišana vse do 12 ur, kar bi lahko bilo posledica nespecifičnega stresnega odgovora celic na vstop amiloidnega peptida v celico. Izpostavitev A[beta](25-35) ne vpliva statistično značilno na množino aktivnega katepsina X v celicah PC-12, saj ostaja kljub povečanem izražanju gena za (pro)katepsina X, množina aktivnega katepsina X znotraj celic bolj ali manj konstantna. Morebitno izločanje aktivnega katepsin X iz celic v gojišče bi lahko potrdili z meritvijo encimske aktivnosti katepsina X v samem gojišču. Modelne celice PC-12 se torej odzivajo na prisotnost A[beta](25-35) v gojišču z nadizražanjem znotrajcelično izmerjenega prokatepsina X, kar bi lahko pomenilo nov molekulski mehanizem v obrambi pred amiloidom.Type of material - master's thesis ; adult, seriousPublication and manufacture - Ljubljana : [B. Šketelj], 2020Language - slovenianCOBISS.SI-ID - 4884593
Author
Šketelj, Blaž
Other authors
Doljak, Bojan
Topics
Cisteinske proteaze |
Imunski odziv |
(pro)katepsin X |
amiloid beta |
celična linija PC-12 |
nevrodegeneracija
Call number – location, accession no. ... |
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Knjižnica mlab 0000000577 ŠKETELJ BLAŽ Izražanje 0000000217 IN: 0016565 Knjižnica mlab 577 ŠKETELJ BLAŽ Izražanje 217 IN: 0016565 |
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Database name | Field | Year |
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Links to authors' personal bibliographies | Links to information on researchers in the SICRIS system |
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Šketelj, Blaž | |
Doljak, Bojan | 19785 |
Source: Personal bibliographies
and: SICRIS
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