The polarization properties of radio sources at very low frequencies (<200 MHz) have not been widely measured, but the new generation of low-frequency radio telescopes, including the Low Frequency ...Array (LOFAR: a Square Kilometre Array Low pathfinder), now gives us the opportunity to investigate these properties. In this paper, we report on the preliminary development of a data reduction pipeline to carry out polarization processing and Faraday tomography for data from the LOFAR Two-meter Sky Survey (LOTSS) and present the results of this pipeline from the LOTSS preliminary data release region (10h45m–15h30m right ascension, 45°–57° declination, 570 square degrees). We have produced a catalog of 92 polarized radio sources at 150 MHz at 4.′3 resolution and 1 mJy rms sensitivity, which is the largest catalog of polarized sources at such low frequencies. We estimate a lower limit to the polarized source surface density at 150 MHz, with our resolution and sensitivity, of 1 source per 6.2 square degrees. We find that our Faraday depth measurements are in agreement with previous measurements and have significantly smaller errors. Most of our sources show significant depolarization compared to 1.4 GHz, but there is a small population of sources with low depolarization indicating that their polarized emission is highly localized in Faraday depth. We predict that an extension of this work to the full LOTSS data would detect at least 3400 polarized sources using the same methods, and probably considerably more with improved data processing.
Marine ecosystems are constantly being threatened by contaminants produced by human activities. There is an urge to better understand their impacts on marine organisms and develop reliable tools for ...biomonitoring studies, while also assessing their potential impacts on human health. Given their position on top of food webs, sharks are particularly susceptible to bioaccumulation, making them potential sentinel species of marine contamination. The main objective of this study was to find suitable biomarkers for future marine pollution biomonitoring studies by correlating biochemical responses with tissue contaminant body burden in blue sharks (Prionace glauca), a species heavily caught and consumed by humans, while also addressing their general health. The chemical contaminants analysed comprised different persistent organic pollutants (POPs) families from polychlorinated compounds to brominated flame retardants (BFRs) and perfluorinated compounds (PFCs) and different trace and heavy metals. Concentrations of some contaminants in sharks' tissues were found to be above the legally allowed limits for human consumption. A canonical correspondence analysis (CCA) was performed and some strong associations were found between biochemical responses and contaminants' accumulation levels. DNA damage and lipid peroxidation levels, as well as the inhibition of the antioxidant enzyme glutathione peroxidase, were the main effects and consequences of contamination. The impact of contamination on these vital macromolecules underlines the suboptimal conditions of the sampled P. glauca, which can ultimately lead to the degradation of core ecological aspects, such as swimming, feeding, and reproduction. It can be concluded that P. glauca demonstrates great potential to be used as environmental sentinel and suitable biomarker candidates were identified in this work. Moreover, this study also highlights the risks that the consumption of blue shark derived products can pose to human health, which is of upmost interest as the sampled organisms were still juveniles and already presented values above regulatory limits.
Display omitted
•Metals and POPs were quantified in the muscle and liver of blue sharks.•Biochemical stress responses were addressed in the same sharks.•Correlations between contaminant levels and biochemical responses were found.•Suitable biomarkers for future pollution biomonitoring studies were proposed.•Sharks presented contamination values above regulatory limits for human consumption.
One of the main challenges of tissue engineering in dentistry is to replace bone and dental tissues with strategies or techniques that simulate physiological tissue repair conditions. This systematic ...review of in vitro studies aimed to evaluate the influence of the addition of nanohydroxyapatite (NHap) to scaffolds on cell proliferation and osteogenic and odontogenic differentiation of human mesenchymal stem cells. In vitro studies on human stem cells that proliferated and differentiated into odontogenic and osteogenic cells in scaffolds containing NHap were included in this study. Searches in PubMed/MEDLINE, Scopus, Web of Science, OpenGrey, ProQuest, and Cochrane Library electronic databases were performed. The total of 333 articles was found across all databases. After reading and analyzing titles and abstracts, 8 articles were selected for full reading and extraction of qualitative data. Results showed that despite the large variability in scaffold composition, NHap-containing scaffolds promoted high rates of cell proliferation, increased alkaline phosphatase (ALP) activity during short culture periods, and induced differentiation, as evidenced by the high expression of genes involved in osteogenesis and odontogenesis. However, further studies with greater standardization regarding NHap concentration, type of scaffolds, and evaluation period are needed to observe possible interference of these criteria in the action of NHap on the proliferation and differentiation of human stem cells.
RAS-pathway mutations are recurrent events in myeloid malignancies. However, there is limited data on the significance of RAS-pathway mutations in patients with myelofibrosis (MF). We analyzed ...next-generation sequencing data of 16 genes, including RAS-pathway genes, from 723 patients with primary and secondary MF across three international centers and evaluated their significance. N/KRAS variants were present in 6% of patients and were typically sub-clonal (median VAF = 20%) relative to other genes variants. RAS variants were associated with advanced MF features including leukocytosis (p = 0.02), high somatic mutation burden (p < 0.01) and the presence of established "molecular high-risk" (MHR) mutations. MF patients with N/KRAS mutations had shorter 3-year overall survival (OS) (34% vs 58%, p < 0.001) and higher incidence of acute myeloid leukemia at 3 years (18% vs 11%, p = 0.03). In a multivariate Cox model, RAS mutations were associated with decreased OS (HR 1.93, p < 0.001). We created a novel score to predict OS incorporating RAS mutations, and it predicted OS across training and validation cohorts. Patients with intermediate risk/high-risk DIPSS with RAS mutations who received ruxolitinib had a nonsignificant longer 2-year OS relative to those who did not receive ruxolitinib. These data demonstrate the importance of identifying RAS mutations in MF patients.
We have synthesized anionic multistimuli responsive core-shell polymer nanoparticles with low size dispersity composed of glassy poly(methyl methacrylate) (PMMA) cores of ca. 40 nm radius and ...poly(N-isopropylacrylamide) (PNIPAM) anionic brush-like shells with methacrylic acid comonomers. Using dynamic light scattering, we observed a volume phase transition upon an increase in temperature and this response was pH and ionic strength dependent. Already at room temperature we observed a pronounced polyelectrolyte effect, that is, a shift of the apparent pKa extracted from the degree of dissociation of the acids as a function of the pH. The multiresponsive behavior of the hydrophobic polyelectrolyte brush has been modeled using the Scheutjens-Fleer self-consistent field (SF-SCF) approach. Using a phenomenological relation between the Flory-Huggins χ parameter and the temperature, we confront the predicted change in the brush height with the observed change of the hydrodynamic radius and degree of dissociation and obtain estimates for the average chain lengths (number of Kuhn segments) of the corona chains, the grafting density and charge density distributions. The theory reveals a rich internal structure of the hydrophobic polyelectrolyte brush, especially near the collapse transition, where we find a microphase segregated structure. Considering this complexity, it is fair to state that the theoretical predictions follow the experimental data semiquantitatively, and it is attractive to attribute the observed disparity between theory and experiments to the unknown polydispersity of the chains, the unknown distribution of the charges, or other experimental complications. More likely, however, the deviations point to significant problems of the mean field theory, which focuses solely on the radial distributions and ignores the possibility of the formation of lateral (local) inhomogeneities in partially collapsed polyelectrolyte brushes. We argue that the PNIPAM brush at room temperature is already behaving nonideally.
The effect of phenol shock loads (100–3000 mg phenol/L) on the performance of an activated-sludge (AS) – moving bed biofilm reactor (MBBR) system was assessed. The AS-MBBR system could well withstand ...phenol shocks up to 500 mg phenol/L (organic load of 2.72 kgCOD/(m3d)), above which its performance was disturbed, more or less intensely depending on the phenol load. Nevertheless, acclimation of microorganisms to the increasing phenol levels was observed, and full phenolic COD removal was reached at 2000 mg phenol/L. Ammonium removal occurred by both bacterial assimilation and nitrification. Nitrifiers showed higher sensitivity to phenol than heterotrophs, being already impaired at 250 mg phenol/L. However, further adaptation of ammonium oxidizers allowed stable ammonium oxidation activity to be reached up to 1500 mg phenol/L. Nevertheless, nitrite oxidizers were severely affected above 250 mg phenol/L, leading to nitrite build-up. At 3000 mg phenol/L, phenol toxicity strongly limited microbial activity. Meanwhile, COD and ammonium removal performance was dramatically impaired. Moreover, the increasing phenol loads led to the development of filamentous organisms, deteriorating the biomass settling properties. The MBBR, employed downstream of the AS reactor, was frequently subjected to low (sometimes nil) phenol loads, not being therefore acclimated to this compound. Once phenol reached this reactor during some shocks, it accounted for less than 23% of the overall COD removal. The results indicated that the use of a non-acclimated biofilm reactor as a polishing step under phenol stress conditions did not always render improvements to the overall treatment and its implementation is only recommended under certain shock loads.
•The effect of phenol shock loads (100–3000 mg/L) on an AS-MBBR system was assessed.•Bacteria acclimation allowed full phenolic COD removal at 2000 mg phenol/L.•Nitrifiers, especially NOB, showed higher sensitivity to phenol than heterotrophs.•At 3000 mg/L, phenol toxicity limited microbial activity and system functioning.•Development of filamentous bacteria at high phenol loads hindered biomass settling.
Previous star formation studies have, out of necessity, often defined a population of young stars confined to the proximity of a molecular cloud.
Gaia
allows us to examine a wider, three-dimensional ...structure of nearby star forming regions, leading to a new understanding of their history. We present a wide-area survey covering 494 deg
2
of the Lupus complex, a prototypical low-mass star forming region. Our survey includes all known molecular clouds in this region as well as parts of the Upper Scorpius and Upper Centaurus Lupus (UCL) groups of the Sco-Cen complex. We combine
Gaia
DR2 proper motions and parallaxes as well as ALLWISE mid-infrared photometry to select young stellar objects (YSOs) with disks. The YSO ages are inferred from
Gaia
color-magnitude diagrams, and their evolutionary stages from the slope of the spectral energy distributions. We find 98 new disk-bearing sources. Our new sample includes objects with ages ranging from 1 to 15 Myr and masses ranging from 0.05 to 0.5
M
⊙
, and consists of 56 sources with thick disks and 42 sources with anemic disks. While the youngest members are concentrated in the clouds and at distances of 160 pc, there is a distributed population of slightly older stars that overlap in proper motion, spatial distribution, distance, and age with the Lupus and UCL groups. The spatial and kinematic properties of the new disk-bearing YSOs indicate that Lupus and UCL are not distinct groups. Our new sample comprises some of the nearest disks to Earth at these ages, and thus provides an important target for follow-up studies of disks and accretion in very low mass stars, for example with ALMA and ESO-VLT X-shooter.
We present a new model to describe DNA interactions with large ligands such as proteins, based on a quenched-disorder equation for ligand binding along the double helix and on Manning's description ...for the local changes of the persistence length at the binding sites. Such a model allows one to extract the physical chemistry of the interactions from pure mechanical measurements, such as those typically performed with DNA-protein complexes in force spectroscopy assays. We have tested the proposed methodology here to investigate the DNA interaction with the protein lysozyme, determining binding parameters such as the equilibrium association constant, the cooperativity degree of the binding reaction, and the fraction of neutralized charges on the phosphate backbone. The model also allows one to get information on the size and positional conformation of the bound proteins.
Fever is a regulated increase of the body temperature resulting from both infectious and non-infectious causes. Fever is known to play a role in modulating immune responses to infection, but the ...potential of febrile temperatures in regulating antigen binding affinity to antibodies has not been explored. Here we investigated this process under in vitro conditions using Isothermal titration calorimetry and ELISA. We used selected malarial and dengue antigens against specific monoclonal antibodies, and observed a marked increase in the affinity of these antibody-antigen complexes at 40°C, compared to physiological (37°C) or pathophysiological temperatures (42°C). Induced thermal equilibration of the protein partners at these temperatures in vitro, prior to measurements, further increased their binding affinity. These results suggest another positive and adaptive role for fever in vivo, and highlight the favourable role of thermal priming in enhancing protein-protein affinity for samples with limited availability.
The edible flowers are widely used, but there is still a lot to be done in relation to its bioactive potential and its correlation with the presence of phenolic compounds. The aim of this study was ...determined the individual phenolic profile in the hydromethanolic extracts and infusion preparations of four different flower samples (Dahlia mignon, Rosa damascena ‘Alexandria’ and R. gallica ‘Francesa’ draft in R. canina, Calendula officinalis L., and Centaurea cyanus L.) and their bioactive potential (antioxidant, antiproliferative, and antibacterial capacity). All the studied flowers presented different profiles regarding their phenolic composition and revealed biological potential. The bioactive potential of the studied flowers was moderate, the hydromethanolic extracts of rose petals showed the best results for antioxidant and antibacterial assays, while the antiproliferative properties were only present in some of the tested cell lines, for the hydromethanolic extracts, in which dahlia and rose showed the best results. These results demonstrate that edible flowers can be used as a source of phenolic compounds with bioactive potential, which can be applied in the food sector, as foods and as sources natural ingredients.
Display omitted
•Edible flowers are a source of phenolic compounds.•Phenolic compounds present in edible flowers are responsible for the bioactivities.•Edible flowers showed antioxidant, antiproliferative, and antibacterial potential.•Rose hydromethanolic extracts showed the best antioxidant and antibacterial results.•Dahlia hydromethanolic extracts showed the highest antiproliferative properties.
PDF
