The occurrence of 93 pharmaceuticals, illicit drugs and their metabolites has been investigated in stabilized sewage sludge from five municipal wastewater treatment plants (WWTPs) in the Slovak ...Republic. The total population connected to the tested WWTPs was approximately 600,000 p.e. which represents >20% of the Slovak population connected to public sewer systems. The sludge production from the five tested plants was >8100tons in 2016, which is approximately 15% of the total Slovak sewage sludge production in 2016.
The highest total concentration of all pharmaceuticals was found in WWTP Bratislava Devínska Nová Ves (DNV) and Senec - 11,800 and 11,300ng/g dry matter (DM), respectively. Among individual pharmaceuticals, the highest concentrations were recorded for fexofenadine (mean 2340ng/g DM, maximum 5600ng/g DM in Bratislava DNV) and telmisartan (mean 1170ng/g DM, with a maximum of 3370ng/g DM in Senec). A principal component analysis revealed differences between pharmaceutical patterns in aerobically and anaerobically stabilized sludge.
The worst-case scenario based on no further degradation of pharmaceuticals between sludge production and field application was used to predict pharmaceutical mass loads in agriculture. For the result, we estimated an annual load to soil in the Slovak Republic of up to several hundred kilograms of pharmaceuticals and drugs, with the maximum for fexofenadine (120kg/year) and verapamil (29kg/year).
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•The first screen of pharmaceuticals and illicit drugs in sewage sludge in Slovakia.•52 target compounds quantified in the investigated samples.•The highest findings for fexofenadine - 5600ng/g DM and telmisartan - 3400ng/g DM•Aerobic sludge differed from anaerobic sludge in the pattern of pharmaceuticals•Estimated pharmaceutical loads to soils reach hundreds of kilograms yearly.
Wastewater contains a wealth of information about the inhabitants of cities. Wastewater-based epidemiology (WBE) has become an effective tool for monitoring public health by analyzing various ...biomarkers (e.g., chemicals and microorganisms) in wastewater. This way, the estimation of pharmaceuticals' consumption behavior and/or illicit drugs can be calculated. However, monitoring consumption alone is not the only option. If we consider wastewater as a statistical representation of the population's health, medical information can be derived. In this work, we used data from 15 different wastewater treatment plants in Slovak Republic to explore correlations between the use of typical pharmaceuticals and illicit drugs. The analysis was based on the wastewater monitoring data from four years (2016–2019), and 68 different compounds were taken into account. One of the strongest correlations found was between Antihyperlipidemics and Antihypertensives, with Pearson's correlation coefficient of 0.82. This type of analysis within the WBE represents a new potential as an additional source of information for the pharmaceutical, medical and government sectors in assessing health risk factors in the population. Such an evaluation method has even a great potential for artificial intelligence and machine learning for calculating health risk factors together with other sources of data.
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•Correlation coefficients between the amounts of detected pharmaceuticals.•Correlations corresponds to the relations between pharmaceutical use.•More than 20% of Slovak population were included in the study.
This contribution reviews the possibility of using the by-products from biodiesel production as substrates for anaerobic digestion and production of biogas. The process of biodiesel production is ...predominantly carried out by catalyzed transesterification. Besides desired methylesters, this reaction provides also few other products, including crude glycerol, oil-pressed cakes, and washing water. Crude glycerol or g-phase is heavier separate liquid phase, composed mainly by glycerol. A couple of studies have demonstrated the possibility of biogas production, using g-phase as a single substrate, and it has also shown a great potential as a cosubstrate by anaerobic treatment of different types of organic waste or energy crops. Oil cakes or oil meals are solid residues obtained after oil extraction from the seeds. Another possible by-product is the washing water from raw biodiesel purification, which is an oily and soapy liquid. All of these materials have been suggested as feasible substrates for anaerobic degradation, although some issues and inhibitory factors have to be considered.
This work presents environmental and quality-control data from the analyses of 33 antibiotics in influent and effluent water from two waste water treatment plants (WWTPs) in the capital and the ...biggest city of Slovakia. Seeing that consumption of antibiotics depends on epidemiological season, samples were collected during February and August. Among assessed antibiotics ciprofloxacin and clarithromycin were detected in highest concentrations in influent water. Seasonal changes were observed only in plant A when antibiotic concentrations decreased. On the other hand an increase in some cases was observed in plant B. Insufficient degradation of some macrolides, sulfonamides and trimethoprim was detected according to their higher concentrations in effluent water. Contact of antibiotics in subinhibitory concentrations and sludge bacteria in WWTPs represent the base for the development of significant levels of microbial resistance. Simultaneously, antibiotic resistance of fecal coliforms and fecal streptococci from sewage sludge was evaluated. Majority of coliform bacteria were found to be resistant to ampicillin and gentamicin. A significant seasonal difference was determined only in case of high-level resistance. In summer samples, an increase in the strains resistant to concentrations higher than the resistance breakpoints established by EUCAST and NCCLS was observed. No antibiotic resistance in streptococci was observed. However, as a part of sewage sludge is mixed with compost and utilized in agriculture, better processing of sludge should be considered.
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•Analysis of selected antibiotics in influent and effluent wastewater in Slovakia.•Determination of antibiotic resistant coliforms prevalence in sewage sludge.•Comparison of seasonal occurrence of antibiotics and resistants in two WWTPs.
Consumption of alcohol and new psychoactive substances (NPS) in a population or during special events (music festivals) is usually monitored through individual questionnaires, forensic and ...toxicological data, and drug seizures. However, consumption estimates have some biases due mostly to the unknown composition of drug pills for NPS and stockpiling for alcohol. The aim of this study was to evaluate for the first time the real use of alcohol and the occurrence of NPS in Slovakia by wastewater-based epidemiology (WBE). Urban wastewater samples were collected from nine Slovak cities over two years (2017–2018) and during three music festivals. The study included about 20% of the Slovak population and 50 000 festival attendees. The urinary alcohol biomarker ethyl sulfate (EtS) and thirty NPS were analyzed by liquid chromatography tandem mass spectrometry (LC – MS/MS). EtS concentrations were used for estimating the per capita alcohol consumption in each city. The average alcohol consumption in the selected cities and festivals in 2017–2018 ranged between 7 and 126 L/day/1000 inhabitants and increased during the weekends and music festivals. Five NPS belonging to the classes of synthetic cathinones (mephedrone, methcathinone, buphedrone and pentedrone) and phenethylamines (25-iP-NBoMe) were found in the low ng/L range. Methcathinone was the most frequently detected NPS, while the highest normalized mass load corresponded to mephedrone (3.1 mg/day/1000 inhabitants). Wastewater-based epidemiology can provide timely information on alcohol consumption and NPS occurrence at the community level that is complementary to epidemiology-based monitoring techniques (e.g. population surveys, police seizures, sales statistics).
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•Monitoring new psychoactive substances and ethyl sulfate in Slovak wastewaters.•30 new psychoactive substances in wastewater during 2 festivals have been searched.•Synthetic cathinones and phenethylamines were primarily detected.•The most detected compound was methcathinone.•Alcohol use was prevalent at dance and multicultural festival.
•The total desorbed amount of drugs is higher from anaerobic than aerobic sludge•Desorbed fractions can be used as a qualitative indicator of sludge/water mobility•Alkaline conditions increased the ...release of pharmaceuticals and illicit drugs•Desorbed fractions cannot be applied for terrestrial toxicity estimation
Pharmaceutical and illicit drug residues in sewage sludge may present important risks following direct application to agricultural soils, potentially resulting in uptake by plants. Leaching/desorption tests were performed on different types of stabilized sewage sludge originating from multiple treatment technologies in the Slovak Republic. Acid rain and base-rich condition of soil with different pH conditions were simulated to model the effect of widely varying pH (pH 2, 4, 7, 9, and 12) on the leaching/desorption of pharmaceuticals and illicit drugs. Twenty-nine of 93 target analytes were found above the limit of quantification in sludge or associated leachates. Total desorbed amounts of pharmaceuticals and illicit drugs ranged from 810 to 4000 µg/kg, and 110 to 3600 µg/kg of the dry mass of anaerobic and aerobic sludge, respectively. Desorbed fractions were calculated as these values are normalized to initial sludge concentration and, therefore, were more suitable for qualitative description of the behavior of individual compounds. Using principal component analysis, qualitative analysis of the desorbed fraction confirmed the differences among sludge types, pharmaceuticals, and desorption pH. Desorbed fractions could not be related to the octanol/water distribution coefficient. Desorbed fractions also did not reflect the expected ionization of studied molecules unless converted into their relative values. Generally, the lowest mobility was observed within the environmentally relevant pH range of 4-9, and high pH generally resulted in high desorption, especially in anaerobically stabilized sludges.
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The aim of this paper is to present a survey on the current status of wastewater treatment systems in 11 central and eastern European (CEE) countries, with a focus on rural areas and on small ...treatment wetlands for settlements of below 2,000 people. The results indicate that CEE countries have insufficient sanitation systems with different performance efficiencies. These differences stem from the different historical, political and economic developments as well as legislation in the previous five to six decades. CEE settlements with less than 2,000 inhabitants represent almost 30.0 % of the overall number of persons living in CEE countries. These data show that CEE countries have a mainly rural character, but this is slowly decreasing. Over 42 million inhabitants are waiting for proper sanitation systems. This requirement will be ever-growing in the next future, and it is important to know and to prepare appropriate steps for progressive realisation.
Drug consumption in individual cities, regions, and at various music events and festivals across the EU has generally been monitored via questionnaires, patients' medical data, and police reports. ...However, an overview of drug consumption obtained from these methods can be negatively affected by various subjective factors. We aimed to investigate an association between levels of target drugs in wastewater, music genres, and festival courses. The occurrence of illicit drugs, their metabolites, and psychoactive compounds was investigated in the influent of six wastewater treatment plants in the Czech and Slovak Republic during seven large-scale music festivals from different music genres: metal, rock, pop, country and folk, ethnic, multi-genre, dance, and trance. The total number of participants included >130,000 active festival attendees. The association between music genre and illicit drug and/or psychoactive pharmaceutical consumptions is discussed on the basis of the results obtained through wastewater analyses. The observed trend was similar to worldwide published data with a specific local phenomenon of methamphetamine prevalence that did not significantly change between music events. Increased specific loads of cocaine (measured as its metabolite benzoylecgonine) and Ecstasy, along with some cannabis, were mainly observed during pop/rock and dance music festivals. However, there was no significant increase observed in the specific loads of all monitored psychoactive pharmaceuticals. This study demonstrates that the abuse of some illicit drugs is closely associated with specific music preferences.
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•26 pharmaceuticals, illicit drugs and their metabolites in wastewater during 7 music festivals have been screened•Ecstasy – dominant drug of dance festival•Cocaine and methamphetamine load in wastewater increased during rock and dance music festival•Cannabis is consumed independently on the music genre preferences.
•Target analysis and suspect screening of 311 new psychoactive substances in wastewater.•One of the largest study in Europe (12 cities, 7 countries) performed in the last years.•Synthetic cathinones ...and arylcyclohexyamines were most stable in wastewater.•Fentanyl, norfentanyl and 15 new psychoactive substances found in wastewater.•The highest mass loads were for 3-methylmethcathinone, especially during weekends.
Wastewater-based epidemiology (WBE) can provide objective and timely information on the use of new psychoactive substances (NPS), originally designed as legal alternatives of internationally controlled drugs. NPS have rapidly emerged on the global drug market, posing a challenge to drug policy and constituting a risk to public health. In this study, a WBE approach was applied to monitor the use of more than 300 NPS, together with fentanyl and its main metabolite norfentanyl, in influent wastewater collected from 12 European cities during March-June 2021. Quantitative and qualitative analysis of NPS in composite 24 h influent wastewater samples were based on solid phase extraction and liquid chromatography-mass spectrometry. In-sample stability tests demonstrated the suitability of most investigated biomarkers, except for a few synthetic opioids, synthetic cannabinoids and phenetylamines. Fentanyl, norfentanyl and eight NPS were quantified in influent wastewater and at least three substances were found in each city, demonstrating their use in Europe. N,N-dimethyltryptamine and 3-methylmethcathinone (3-MMC) were the most common NPS found, with the latter having the highest mass loads (up to 24.8 mg/day/1000 inhabitants). Seven additional substances, belonging to five categories of NPS, were identified in different cities. Spatial trends of NPS use were observed between cities and countries, and a changing weekly profile of use was observed for 3-MMC. WBE is a useful tool to rapidly evaluate emerging trends of NPS use, complementing common indicators (i.e. population surveys, seizures) and helping to establish measures for public health protection.
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