Tehnički razvoj javnoga radija u Hrvatskoj Šimunović, Damir; Bujić, Nikolina; Fajt, Siniša
Annual of the Croatian Academy of Engineering,
05/2020, Volume:
2019, Issue:
1
Journal Article
Open access
Radio Zagreb, začet u radioamaterskom klubu, započeo je svoj pro-fesionalni put 1926. godine. Njegova tehnička ekipa kroz cijelu je povijest postojanja nastojala slijediti tehnološki napredak, ...programskoj ekipi osigu-rati suvremene uvjete rada, a slušateljima kvalitetan prijem, usprkos brojnim političkim i financijskim preprekama i previranjima. Od jedne stanice sa sla-bašnim srednjevalnim odašiljačem do mreže Hrvatskoga radija s tri nacio-nalna, osam regionalnih i jednim internacionalnim programom u zemaljskoj, digitalnoj satelitskoj, kabelskoj i internetskoj distribuciji trebalo je prihvatiti niz promjena u tehnologijama, izgrađivati nove studije i reportažna kola, mi-jenjati standarde, a ipak održati kontinuitet. Ovim se tekstom želimo podsje-titi na brojne inženjere i tehničare koji su to ostvarili.
Tehnički razvoj javne televizije u Hrvatskoj Šimunović, Damir; Bujić, Nikolina; Fajt, Siniša
Annual of the Croatian Academy of Engineering,
05/2020, Volume:
2019, Issue:
1
Journal Article
Open access
Na tada več vrlo stabilnim temeljima Radio Zagreba, 1956. je na-stala Televizija Zagreb, danas Hrvatska televizija. Okolnosti joj nisu omogu-ćile da nastane ranije, iako je snažna želja, osobito u ...tehničkim krugovima, postojala. Napokon su se tih godina stekli uvjeti za investicije i razvoj koji je, kada je napokon krenuo, bio nezaustavljiv. Iako se susretala s još većim po-teškoćama nego stariji brat radio zbog svojih većih zahtjeva na prostor i ljudske resurse, skuplje tehnologije i općenito skuplje produkcije, televizija je kroz dugi niz godina imala veliku podršku publike. Prijelomni trenutak bilo je preseljenje na Prisavlje u velike studije te ulaganja u terensku tehniku što je omogućilo realizaciju najzahtjevnih programskih projekata u ovom dijelu Eu-rope. Ovaj tekst odaje priznanje ljudima koji su to ostvarili.
Limbal Stem Cell Deficiency (LSCD) is a very serious and painful disease that often results in impaired vision. Cultivation of limbal stem cells for clinical application is usually performed on ...carriers such as amniotic membrane or surgical fibrin gel. Transplantation of these grafts is associated with the risk of local postoperative infection that can destroy the graft and devoid therapeutic benefit. For this reason, electrospun scaffolds are good alternatives, as proven to mimic the natural cells surroundings, while their fabrication technique is versatile with regard to polymer functionalization and scaffolds architecture. This study considers the development of poly(ε-caprolactone) (PCL) immune-compatible and biodegradable electrospun scaffolds, comprising cefuroxime (CF) or titanium dioxide (TiO2) active components, that provide both bactericidal activity against eye infections and support of limbal stem cells growth in vitro. The PCL/CF scaffolds were prepared by blend electrospinning, while functionalization with the TiO2 particles was performed by ultrasonic post-processing treatment. The fabricated scaffolds were evaluated in regard to their physical structure, wetting ability, static and dynamic mechanical behaviour, antimicrobial efficiency and drug release, through scanning electron microscopy, water contact angle measurement, tensile testing and dynamic mechanical analysis, antimicrobial tests and UV-Vis spectroscopy, respectively. Human limbal stem cells, isolated from surgical remains of human cadaveric cornea, were cultured on the PCL/CF and PCL/TiO2 scaffolds and further identified through immunocytochemistry in terms of cell type thus were stained against p63 marker for limbal stem cells, a nuclear transcription factor and cytokeratin 3 (CK3), a corneal epithelial differentiation marker. The electrospun PCL/CF and PCL/TiO2 successfully supported the adhesion, proliferation and differentiation of the cultivated limbal cells and provided the antimicrobial effect against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans.
PDF