This study aimed to evaluate the discrepancies between genetic and visual coat color assignment in the Sarcidano Horse and to elucidate potential reasons. Individual DNA from 90 Sarcidano Horses was ...used for genetic assignment of coat color to explore the correspondence with individual forms containing phenotypical traits. The
exon 1 and
exon 3 have been genotyped and sequenced to obtain a picture of the coat color distribution in this breed. Surprisingly, once we compared the genetic results with the individual forms reporting the phenotypic data for each subject, a certain degree of non-correspondence between the phenotypic and genetic data in relation to coat color emerged. From the genetic analysis, Chestnuts (
= 58) resulted the most common Sarcidano Horse (
= 58), followed by a quite large number of Blacks (
= 28) and a very small number of Bays (
= 4), whereas phenotypic distribution resulted in 38 Chestnuts, 40 Bays, only 2 Blacks, and 10 Grays (without the possibility of recognizing the true color they carried). Chestnut resulted a very representative coat color, while many horses that visually identified as Bays were genetically Blacks. This discrepancy, that could be due to a variety of individual and external factors, including age, time of year, living situation and dietary condition, suggesting the importance of accurate coat color identification to ensure adequate features registration and reliable prediction of offspring's coat color.
The aim of the present research was to evaluate the association between the SNPs rs430181568 and rs407388227 located in the MTNR1A gene with the reproductive recovery of Sarda sheep in different ...months of ram introduction in the flock (February, March, April and May). In order to address this research gap, we selected two farms, each of which consisted of approximately 1000 animals; a total of 800 ewes (400 for each farm) were genotyped for the two single nucleotide polymorphisms rs430181568 and rs407388227 located in the exon 2 of the MTNR1A. These SNPs are completely linked; thus, each genotype of rs430181568 corresponded to the same genotype for rs407388227. Among the genotyped animals, 240 individuals were selected and divided into four homogeneous groups (A, B, C and D) of 60 subjects, each group based on age (range 3−6 years old), body condition score (BCS) (range 2.0−4.0) and genotype (20 ewes carrying CC/CC, 20 CT/CT and 20 TT/TT genotype). The dates of the ram introduction in each group were 15 February, 15 March, 15 April and 15 May, respectively. In all groups, the lambing date and the number of lambs born from 150 to 220 days after the ram introduction were recorded. In all the groups, the genotypes CC/CC and CT/CT of the polymorphism (rs430181568 and rs407388227) showed the greatest fertility (the ratio between the number of lambed ewes and the ewes exposed to the rams) (p < 0.01) and the shortest distance between ram introduction to lambing (p < 0.01), compared with the TT/TT genotype. In conclusion, we determined that the polymorphisms rs430181568 and rs407388227 were associated with reproductive recovery, after ram introduction, even in February and March, months subsequent to the photorefractoriness period.
Melatonin receptor MT1, encoded by the
gene, is the main one involved in the seasonal regulation of reproductive activity. A correlation between this gene polymorphism and reproductive performance ...has been demonstrated in sheep. To date, no data about
gene polymorphism are available regarding Italian goat breeds other than the Sarda goat. This study aimed to detect any PCR-RFLP polymorphic sites of
using
and
enzymes in Northern Italian goat breeds, which are characterized by a pronounced reproductive seasonality. One-hundred-eight adult female goats belonging to four different breeds were included in the study (i.e., Frisa Valtellinese,
= 29; Orobica,
= 23; Lariana,
= 29; Camosciata delle Alpi,
= 27). Blood was sampled from each goat. Genomic DNA was extracted from each sample and the main part of exon II of
gene was amplified by PCR and digested with
and
enzymes. Unexpectedly, none of the fragments were found to be polymorphic. The absence of polymorphism may be linked to the macro group of goat breeds that evolved during human migrations. Breeds of the Alpine-European strain would appear to show no polymorphism, as confirmed by our study, whereas breeds belonging to the Mediterranean-African or Asian-Middle Eastern strains do.
•IGF-1 gene plays an important role in regulating reproduction in ruminants.•Here three SNPs were found in the IGF-I gene in Sarda sheep.•The found SNPs were related to reproductive performance and ...milk yield.•This results could be used to improve the reproductive performances and milk yield in Sarda sheep.
The aim of this research was to detect polymorphisms within the IGF-I gene in dairy sheep and to verify their influence on milk yield and reproductive performances. Four hundred Sarda ewes in their second lactation were selected from 2 farms. Their reproductive traits (fertility rate, interval in days from ram introduction to lambing and litter size) and milk yield were recorded, from the second to the fourth lactation. DNA was extracted from individual blood samples and subjected to amplification and sequencing of the IGF-I gene 5′ UTR and Exon 3 regions. Three polymorphic sites were recorded at positions g184028491C>G and g184028489C>T of the 5′UTR, and g184023223G>A of the Exon 3. The C allele at position g184028491 showed a significant association with higher fertility rate (P < 0.05) and a shorter interval in days from ram introduction to lambing (P < 0.01). In addition, a significant effect of the CC genotype was found with higher milk yield for P < 0.05 in the second and third lactation, and P < 0.01 in the fourth lactation compared to the other genotypes. Even, AA genotype at position g184023223 of the exon 3 showed a positive significant effect on milk yield for P < 0.05, in the second and third lactation, and for P < 0.01 in fourth lactation compared to the other genotypes. In conclusion the found SNPs showed a significant influence on reproductive performances and milk yield in Sarda sheep breed suggesting a possible application in sheep selection plans.
The economy in Mediterranean areas is tightly linked to the evolution of the sheep-farming system; therefore, improvement in ewe's reproductive performance is essential in the developing countries of ...this area.
is the gene coding for Melatonin receptor 1 (MT1), and it is considered to be involved in the reproductive activity in sheep. The aims of this study were: (1) identifying the polymorphisms from the entire
coding region and promoter in Lebanese Awassi sheep flocks, and (2) investigating the association between the found polymorphisms and the reproductive performance, assessed as lambing rate, litter size, and days to lambing (DTL). The study was conducted in two districts of Lebanon, where 165 lactating ewes, aged 5.2 ± 1.5 years, with body condition score (BCS) 3.3 ± 0.4, were chosen and exposed to adult and fertile rams. From 150 to 220 days after ram introduction, lambing dates and litter sizes were registered. This study provided the entire coding region of the
receptor gene in the Awassi sheep breed. Thirty-one single nucleotide polymorphisms (SNPs) were detected, five of which were missense mutations. The H2, H3, and H4 haplotypes were associated with lower DTL (
< 0.05), as well as the SNPs rs430181568 and rs40738822721, named from now on SNP20 and SNP21, respectively. These SNPs were totally linked and can be considered as a single marker. The ewes carrying the C allele at both these polymorphic sites advanced their reproductive recovery (
< 0.05). These results are essential for improving reproductive management and obtaining advanced lambing in Awassi ewes.
In ruminants, the role of melatonin in the control of reproductive seasonality is well reported, but it is still little known about its action on milk traits and on the immune system. The aim of this ...study was to assess the effect of melatonin on milk yield and composition, somatic cell count (SCC), some cytokine blood concentration and reproductive resumption in sheep. One hundred lactating sheep were allocated to two groups (of 50 sheep each), M (treated with melatonin) and C (controls), and exposed to the rams for 50 d. Time period in days from ram introduction to lambing (TRIL) and litter size were recorded. Every 15 d, from 1 March to 30 April, the individual daily milk yield was registered and milk composition and SCC, were analysed. The levels of Interleukin 2 and 6 (IL-2 and 6) and tumour necrosis factor-alpha (Tnf-α) were evaluated every 15 d. The highest fertility rate (p < .01) and the shortest TRIL (p < .05) was recorded in M group. Milk yield and composition were similar between groups. The somatic cells decreased continuously in the treated ewes (p < .01) and increased continuously in untreated ewes (p < .05). IL-2 and IL-6 increased in M group, although no statistical differences were found between groups. Melatonin administration improved reproductive efficiency, did not affect milk yield and composition and decreased somatic cells. This last effect could help to maintain healthy mammary gland and encourage farmers to reduce the use of drugs against mastitis.
Highlights
Melatonin is involved in different physiological processes, such as the control of reproductive seasonality, milk production and the immune response.
Melatonin administration in Sarda sheep did not modify milk yield and composition, decreased SCC and advanced reproductive resumption in spring.
Melatonin could be used to strengthen the sheep immune response in order to reduce the use of drugs for both therapeutic and preventive purposes against mastitis.
This study aims to find reliable strategies for advancing first mating and shortening the period from weaning to first lambing in ewe lambs. Sarda ewe lambs (n = 400) were selected from two farms and ...allocated into four separated groups of 100, all of which were exposed to fertile, adult rams over the course of a 50-day breeding period. The first treatment group (RMR) was exposed to four melatonin-treated rams which were replaced every ten days, whilst the second treatment group (RM) was exposed to four melatonin-treated rams which were not replaced. Alternatively, the first control group (RCR) was exposed to four untreated rams which were replaced every ten days, whilst the second control group (RC) was exposed to four untreated rams which were not replaced. In each group, lambing dates, fertility rate, litter size, and distance in days from ram introduction to lambing (DRIL) were recorded. The highest fertility rate was recorded in the RMR group (
≤ 0.05). Shorter DRIL (
≤ 0.01) and higher lambing concentrations were recorded in the RM and RMR groups as compared to the controls. The findings indicate that melatonin treatment of rams and their replacement at 10-day intervals results in earlier onset of first mating, increased fertility rate in ewe lambs, and a higher number of ewes that lambs in a shorter time frame.
The goal of this study was to contribute to the general knowledge of the Sarcidano Horse, both by the identification of the genetic basis of the coat color and by updating the exact locations of the ...genotyping sites, based on the current EquCab3.0 genome assembly version. One-hundred Sarcidano Horses, living in semi-feral condition, have been captured to perform health and biometric checks. From that total number, 70 individual samples of whole blood were used for DNA extraction, aimed to characterize the genetic basis of the coat color. By genotyping and sequencing analyses of the MC1R Exon 1 and ASIP Exon 3, a real image of the coat color distribution in the studied population has been obtained. Chestnut and Black resulted in the most representative coat colors both from a phenotypic and genotypic point of view, that is suggestive of no human domestication or crossbreeding with domestic breed. Due to its ancient origin and genetic isolation, an active regional plan for the conservation of this breed would be desirable, focused on maintenance of resident genotypes and genetic resources. Collection and management of DNA, sperm, embryos, with the involvement of research centers and Universities, could be a valid enhancing strategy.
Stakeholders place great emphasis upon rationalizing the management and rearing techniques which are utilized within sheep farms. The present study aimed to investigate factors which may improve the ...reproductive performance of melatonin-treated Sardinian sheep via a series of three trials. The first trial (n = 100) investigated the effect of melatonin treatment alongside body condition score (BCS), the second trial (n = 150) investigated the effect of treatment alongside the date of treatment (treatment period) and the third trial (n = 150) investigated the effect of treatment alongside the previous lambing of the ewes. The findings indicated that melatonin is an effective tool for anticipating and improving the reproductive activity of in Sarda breed sheep during the springtime. Furthermore, to obtain optional results, melatonin implantation should be conducted in April, in ewes that have a BCS of >2.5 and that have passed their third month of lactation.
The object was to evaluate the effect of melatonin treatment on the advance in April of the reproductive resumption in Sarda breed sheep with different lambing period. For the research two farms, ...located in North Sardinia between 39degrees and 40degrees N, were chosen. In each farm, 120 lactating ewes were selected: 30 lambed between October 20th and November 20th (group 1); 30 lambed between December 1st and 30th (group 2); 30 lambed between January 1st and 30th; 30 lambed between February 1st and 28th (group 4). In each farm, each group of 30 animals was divided into two subgroups of 15 animals (M and C). On April 1st, in each farm, the animals of the M subgroups were treated with a implant containing 18 mg melatonin. The subgroups C were kept as control. The lambing dates and the number of newborn lambs were recorded until 220 days after ram introduction. In treated animals greatest fertility (P<0.01) and lowest distance in days from male introduction to lambing (P<0.01) were recorded. The best reproductive performances were found in the group 1 and 2 compared to the other two groups (P<0.01).The present research shows that melatonin treatment should be made 3 or 4 months after lambing, in order to obtain optimal results.
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