Sub-project 5 of BIOEXPLOIT aims to design durable disease resistance through marker-assisted breeding by converting existing markers for high-throughput application, developing and validating ...high-throughput marker technologies and pyramiding major R genes and/or quantitative trait loci into elite material. Activities include (1) the fine mapping of the quantitative trait locus PiXspg which accounts for a large proportion of the variation in late blight resistance, (2) converting SNP-based markers and an AFLP marker to easy-to-use-markers, (3) testing of progenies with combined sources of late blight resistance for presence of R genes and agronomic features, (4) backcrossing new sources of resistance to S. tuberosum and molecular screening of breeding materials with marker GP94 linked with gene Rpi-phu1 conferring late blight resistance, (5) evaluating potato clones with enhanced resistance against Phytophthora infestans under field conditions of Toluca (México), and (6) developing populations and marker-assisted breeding for disease resistance.
In this study, eight transformed Solanum tuberosum L. plants, affected in their nitrate assimilatory pathway by the introduction of a tobacco nitrate reductase gene (Nia2), were cultivated in ...glasshouse conditions at INRA Ploudaniel (West Brittany, France). Two irrigation regimes were compared and plants were sampled at four stages of vegetation. Yield, tuber dry matter content, total nitrogen content, nitrate content in the whole plant, and nitrate reductase activities were studied. High frequency irrigation with nutritive solutions negatively affects both yield and dry matter content in tubers. Moreover, the introduction of the tobacco Nia2 gene in the potato genome does not seem to affect the agronomical parameters of the initial genotype apart from the nitrate content of tubers. Five transgenic genotypes out of eight, in fact, showed a drastic decrease (of around 98%) in their tuber nitrate content. This nitrate decrease in the tubers was also correlated with the presence of the mRNA transgene, whereas the potato nitrate reductase transcript does not seem to be expressed in wild-type tubers. Regarding these genotypes, developmental stage and nutritive solution supply were found to have no effect on tuber nitrate content. In fact, tubers derived from these clones exhibited low nitrate content throughout the vegetation period, while nitrate accumulation in wild-type tubers is progressive and increases sharply with high nutritive solution supply.
The fruit, vegetable and potato (FLP) sectors are characterised by a high structural diversity linked to the many species and varieties cultivated and to very varied production and marketing ...structures. The very strong competition imposed by the long distribution and import-export channels has gradually led, for reasons of economic rationality, to a specialisation of the production basins and part of the farms with standardisation of production means and products, but also the disappearance of many small farms. Overall, these changes have resulted in an erosion of surfaces and a decrease in the volumes produced for the majority of sectors with the exception of potatoes and certain nuts. Nevertheless, the dynamism of certain "product" sectors has led to a revival of competitiveness and/or a better Plénet D. et al. 80 Innovations Agronomiques 68 (2018), 79-105 development of the offer thanks to approaches relating to the development of high-tech systems, the reduction in the use of synthetic products, segmentation and commercial differentiation, and the creation of quality signs. In addition, the renewal of short and local circuits has recently made it possible to develop a very diversified network of farms in all regions. The emergence of high expectations for organic farming products and for healthy and sustainable agro-food systems offers new opportunities today. To benefit from them, the FLP sectors will have to develop coherent strategies to strengthen complementarities and synergies between different production, processing and marketing systems. These diversity enhancement strategies will contribute to increasing the economic and environmentalrobustness of the FLP sectors.
Les filières fruits, légumes et pomme de terre (FLP) se caractérisent par une diversité structurelle importante liée aux nombreuses espèces et variétés cultivées et à des structures de production et de commercialisation très variées. La très forte concurrence imposée par les circuits longs de la grande distribution et de l’import-export a progressivement entrainé pour des raisons de rationalité économique une spécialisation des bassins de production et d’une partie des exploitations avec une standardisation des moyens de production et des produits, mais aussi la disparition de nombreuses petites exploitations. Globalement, ces évolutions se sont traduites par une érosion des surfaces et une diminution des volumes produits pour la majorité des filières à l’exception de la pomme de terre et de certains fruits à coque. Néanmoins, le dynamisme de certaines filières « produit » a entrainé un regain de compétitivité et/ou une meilleure valorisation de l’offre grâce à des démarches portant sur le développement de systèmes à haute technicité, la réduction d’utilisation des produits de synthèse, la segmentation et la différenciation commerciale, et la création de signes de qualité. Par ailleurs, le renouveau des circuits courts et de proximité permet depuis peu de développer dans toutes les régions un tissu d’exploitations très diversifiées. L’émergence d’une forte attente pour les produits de l’agriculture biologique et pour des systèmes agro-alimentaires sains et durables offre aujourd’hui de nouvelles opportunités. Pour en bénéficier, les filières FLP devront élaborer des stratégies cohérentes afin de renforcer les complémentarités et les synergies entre différents systèmes de production, de transformation et de commercialisation. Ces stratégies de valorisation de la diversité contribueront à accroitre la robustesse économique et environnementale des filières FLP.
Developing a core collection consists in selecting, in a more or less large collection, a small panel of genotypes representing the genetic diversity available in the initial collection, with minimum ...redundancy. This study presents the development of a core collection of 48 genotypes representative of the genetic diversity of a panel of 350 tetraploid varieties of potato (Solanum tuberosum L.). It was built using microsatellite marker genotyping data (SSR) and M strategy which maximizes allelic diversity. This core collection forms a panel of major interest that has been exploited up till now in the framework of two research projects: on the one hand to assess the possibility of exploiting the SNP SolCAP array to characterize the genetic resources maintained in BRC BrACySol, and on the other hand, to assess the structural and morphological variability of potato starch grains.
Le développement d’une core collection consiste à sélectionner, au sein d’une collection ayant un effectif plus ou moins important, un petit panel de génotypes qui représente la diversité génétique disponible dans la collection de départ, avec un minimum de redondance. Cette étude présente le développement d’une core collection de 48 génotypes, représentative de la diversité génétique d’un panel de 350 variétés tétraploïdes de pomme de terre (Solanum tuberosum L.). Elle a été établie en utilisant des données de génotypage par marqueurs microsatellites (SSR) et la stratégie M qui maximise la diversité allélique. Cette core collection constitue un panel d’un grand intérêt qui a été exploité jusqu’à présent dans le cadre de deux projets de recherche : d’une part, pour évaluer la possibilité d’exploiter la puce SNP SolCAP pour caractériser les ressources génétiques maintenues au sein du CRB BrACySol, et, d’autre part, pour évaluer la variabilité structurale et morphologique des grains d’amidon de pomme de terre
Imeglimin is the first in a new class of oral glucose-lowering agents currently in phase 2b development. Although imeglimin improves insulin sensitivity in humans, the molecular mechanisms are ...unknown. This study used a model of 16-week high-fat, high-sucrose diet (HFHSD) mice to characterize its antidiabetic effects. Six-week imeglimin treatment significantly decreased glycemia, restored normal glucose tolerance, and improved insulin sensitivity without modifying organs, body weights, and food intake. This was associated with an increase in insulin-stimulated protein kinase B phosphorylation in the liver and muscle. In liver mitochondria, imeglimin redirects substrate flows in favor of complex II, as illustrated by increased respiration with succinate and by the restoration of respiration with glutamate/malate back to control levels. In addition, imeglimin inhibits complex I and restores complex III activities, suggesting an increase in fatty acid oxidation, which is supported by an increase in hepatic 3-hydroxyacetyl-CoA dehydrogenase activity and acylcarnitine profile and the reduction of liver steatosis. Imeglimin also reduces reactive oxygen species production and increases mitochondrial DNA. Finally, imeglimin effects on mitochondrial phospholipid composition could participate in the benefit of imeglimin on mitochondrial function. In conclusion, imeglimin normalizes glucose tolerance and insulin sensitivity by preserving mitochondrial function from oxidative stress and favoring lipid oxidation in liver of HFHSD mice.
Agricultural practices significantly affect soil biodiversity and functions, altering biogeochemical cycles and potentially compromising food production. Increased employment of sustainable ...agricultural practices is of growing policy concern and requires a better understanding and quantification of how agriculture affects soil functioning. We conducted a worldwide meta-analysis by computing 4855 effect sizes from 103 publications to quantify the effect of agricultural practices on soil nematodes, known to be key biological indicators of soil health. Our meta-analysis summarized the effects of tillage, pesticides use, fertilization, manipulation of above-ground plant including cover crop, rotation and agricultural system shift (the conversion from the conventional to conservation or organic agriculture systems). We quantified how each agricultural practice alters nematode indices of ecological relevance including the absolute abundance of trophic groups, the taxonomic richness and diversity and the food web structure based on functional guilds. At the global level, organo-mineral fertilization, conservation system, cover crop and nematicides exhibited the greatest effect sizes (averaged all nematode indices) while herbicides, plant association, mineral fertilization and tillage had the lowest ones. At the level of trophic groups, the agricultural practices had varying impacts, e.g. crop rotation mainly reduced the abundance of the plant-feeding nematodes (−47%), cover crop mainly increased the abundance of omnivore-predators (+80%) while organic fertilization predominately promoted bacterial (+113%) and fungal feeders (+141%). Crop rotation reduced the absolute abundance of plant feeders by 47% when the rotation is longer than 2 years. At the community level, chemical inputs, monoculture and pesticide application reduced nematode abundance, the food web structure and favoured copiotrophic nematode communities. Biocides and nematicides reduced total abundance, Shannon diversity and the food web complexity of soil nematode (structure index). Using meta-regressions, our meta-analysis revealed that the effect of agricultural practices depends on the time since the last agricultural intervention (e.g. input of fertilizers, pesticide application) and on how long a practice has been adopted. This study will be a useful aid for decision maker to better manage soil nematode community and to identify gaps in current available literature. In providing the direction and magnitude of soil nematode responses to agricultural practices, the effect size produced by this study are critical in facilitating worldwide modelling of soil biodiversity under global change scenarios.
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•The worldwide effects of agricultural practices on soil nematodes were quantified.•Rotation, cover crop, organic fertilization and biocides practices had the greatest effects.•Conversion from conventional to conservation agriculture increased nematode abundance and diversity.•Time since the last agricultural intervention and initial nematode abundance were identified as key moderators.•These data could help to model biodiversity under global change scenarios.
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