We report on the deepest X-ray observation of the narrow-line Seyfert 1 galaxy Mrk 335 in the low-flux state obtained with Suzaku. The data are compared to a 2006 high-flux Suzaku observation when ...the source was ∼10 times brighter. Describing the two flux levels self-consistently with partial covering models would require extreme circumstances, as the source would be subject to negligible absorption during the bright state and 95 per cent covering with near Compton-thick material when dim. Blurred reflection from an accretion disc around a nearly maximum spinning black hole (a > 0.91, with preference for a spin parameter as high as ∼0.995) appears more likely and is consistent with the long-term and rapid variability. Measurements of the emissivity profile and spectral modelling indicate the high-flux Suzaku observation of Mrk 335 is consistent with continuum-dominated, jet-like emission (i.e. beamed away from the disc). It can be argued that the ejecta must be confined to within ∼ 25r
g if it does not escape the system. During the low-flux state, the corona becomes compact and only extends to about 5r
g from the black hole, and the spectrum becomes reflection dominated. The low-frequency lags measured at both epochs are comparable indicating that the accretion mechanism is not changing between the two flux levels. Various techniques to study the spectral variability (e.g. principal component analysis, fractional variability, difference spectra, and hardness ratio analysis) indicate that the low-state variability is dominated by changes in the power-law flux and photon index, but that changes in the ionization state of the reflector are also required. Most notably, the ionization parameter becomes inversely correlated with the reflected flux after a long-duration flare-like event during the observation.
Background
Childhood asthma comprises different phenotypes with complex pathophysiology. Different asthma phenotypes evoke various clinical symptoms and vary in their responses to treatments.
Methods
...We applied k‐means clustering algorithm of twelve objective laboratory tests among 351 asthmatic children enrolled in the Taiwanese Consortium of Childhood Asthma Study (TCCAS). We constructed gene expression profiles of peripheral blood mononuclear cells (PBMC) from children with different asthma phenotypes.
Results
Five distinct phenotypes of childhood asthma were identified and can be characterized by either eosinophil‐predominant or neutrophil‐predominant inflammatory characteristics. In the gene expression profile analysis, significant differences were noted for neutrophil‐predominant asthma, compared with samples from all the other asthma phenotypes. The vast majority of the differentially expressed genes in neutrophil‐predominant asthma was associated with corticosteroid response. From an independent inhaled corticosteroid (ICS) response cohort, we also found neutrophils could be activated in this severe asthma phenotype and neutrophil‐predominant asthma may be associated with corticosteroid nonresponsiveness.
Conclusion
Phenotype clustering of childhood asthma can be helpful to identify clinically relevant patients and reveal different inflammatory characteristics in asthmatic children. Neutrophil‐predominant asthma is the most severe asthma phenotype with poor corticosteroid response. Gene expression profile of different asthma phenotypes not only improve our knowledge of childhood asthma, but also can guide asthma precision medicine.
Neutrophil‐predominant asthma is the most severe asthma phenotype with poor corticosteroid response. Five distinct phenotypes of childhood asthma identified in this study with differences in lung function, symptom frequency, healthcare utilization, percentages of eosinophils and neutrophils in peripheral blood, and serum IgE. Gene expression signature in PBMC constitutes an easier way to objectively identify corticosteroid‐resistant asthma in clinical settings.
The optical design and performance of the recently opened 13A biological small‐angle X‐ray scattering (SAXS) beamline at the 3.0 GeV Taiwan Photon Source of the National Synchrotron Radiation ...Research Center are reported. The beamline is designed for studies of biological structures and kinetics in a wide range of length and time scales, from angstrom to micrometre and from microsecond to minutes. A 4 m IU24 undulator of the beamline provides high‐flux X‐rays in the energy range 4.0–23.0 keV. MoB4C double‐multilayer and Si(111) double‐crystal monochromators (DMM/DCM) are combined on the same rotating platform for a smooth rotation transition from a high‐flux beam of ∼4 × 1014 photons s−1 to a high‐energy‐resolution beam of ΔE/E ≃ 1.5 × 10−4; both modes share a constant beam exit. With a set of Kirkpatrick–Baez (KB) mirrors, the X‐ray beam is focused to the farthest SAXS detector position, 52 m from the source. A downstream four‐bounce crystal collimator, comprising two sets of Si(311) double crystals arranged in a dispersive configuration, optionally collimate the DCM (vertically diffracted) beam in the horizontal direction for ultra‐SAXS with a minimum scattering vector q down to 0.0004 Å−1, which allows resolving ordered d‐spacing up to 1 µm. A microbeam, of 10–50 µm beam size, is tailored by a combined set of high‐heat‐load slits followed by micrometre‐precision slits situated at the front‐end 15.5 m position. The second set of KB mirrors then focus the beam to the 40 m sample position, with a demagnification ratio of ∼1.5. A detecting system comprising two in‐vacuum X‐ray pixel detectors is installed to perform synchronized small‐ and wide‐angle X‐ray scattering data collections. The observed beamline performance proves the feasibility of having compound features of high flux, microbeam and ultra‐SAXS in one beamline.
The optical design and performance of the BioSAXS beamline at the Taiwan Photon Source are reported
The narrow-line Seyfert 1 galaxy IRAS 13224−3809 has been observed with XMM-Newton for 500 ks. The source is rapidly variable on time-scales down to a few 100 s. The spectrum shows strong broad Fe − ...K
and L emission features which are interpreted as arising from reflection from the inner parts of an accretion disc around a rapidly spinning black hole. Assuming a power law emissivity for the reflected flux and that the innermost radius corresponds to the innermost stable circular orbit, the black hole spin is measured to be 0.989 with a statistical precision better than 1 per cent. Systematic uncertainties are discussed. A soft X-ray lag of 100 s confirms this scenario. The bulk of the power-law continuum source is located at a radius of 2-3 gravitational radii.
Recent developments in the instrumentation and data analysis of synchrotron small‐angle X‐ray scattering (SAXS) on biomolecules in solution have made biological SAXS (BioSAXS) a mature and popular ...tool in structural biology. This article reports on an advanced endstation developed at beamline 13A of the 3.0 GeV Taiwan Photon Source for biological small‐ and wide‐angle X‐ray scattering (SAXS–WAXS or SWAXS). The endstation features an in‐vacuum SWAXS detection system comprising two mobile area detectors (Eiger X 9M/1M) and an online size‐exclusion chromatography system incorporating several optical probes including a UV–Vis absorption spectrometer and refractometer. The instrumentation and automation allow simultaneous SAXS–WAXS data collection and data reduction for high‐throughput biomolecular conformation and composition determinations. The performance of the endstation is illustrated with the SWAXS data collected for several model proteins in solution, covering a scattering vector magnitude q across three orders of magnitude. The crystal‐model fittings to the data in the q range ∼0.005–2.0 Å−1 indicate high similarity of the solution structures of the proteins to their crystalline forms, except for some subtle hydration‐dependent local details. These results open up new horizons of SWAXS in studying correlated local and global structures of biomolecules in solution.
A new endstation for biological small‐ and wide‐angle X‐ray scattering is detailed, which provides development opportunities for studying correlated local and global structures of biomolecules in solution.
This paper presents an improved genetic algorithm with multiplier updating (IGA/spl I.bar/MU) to solve power economic dispatch (PED) problems of units with valve-point effects and multiple fuels. The ...proposed IGA/spl I.bar/MU integrates the improved genetic algorithm (IGA) and the multiplier updating (MU). The IGA equipped with an improved evolutionary direction operator and a migration operation can efficiently search and actively explore solutions, and the MU is employed to handle the equality and inequality constraints of the PED problem. Few PED problem-related studies have seldom addressed both valve-point loadings and change fuels. To show the advantages of the proposed algorithm, which was applied to test PED problems with one example considering valve-point effects, one example considering multiple fuels, and one example addressing both valve-point effects and multiple fuels. Additionally, the proposed algorithm was compared with previous methods and the conventional genetic algorithm (CGA) with the MU (CGA/spl I.bar/MU), revealing that the proposed IGA/spl I.bar/MU is more effective than previous approaches, and applies the realistic PED problem more efficiently than does the CGA/spl I.bar/MU. Especially, the proposed algorithm is highly promising for the large-scale system of the actual PED operation.
•Nurses still have low to moderate levels of disaster preparedness.•Nurses continue their need for better education to improve disaster preparedness.•It is also essential to enhance psychological ...preparedness of nurses to disasters.•Advanced learning and teaching methods such as high-fidelity simulation is needed.•Need more RCT to examine training effects of nurses’ psychological preparedness.
Disasters and the magnitude of destruction are increasing worldwide. Nurses constitute the largest number of healthcare providers and have major roles in disaster response and care. They need to have sufficient knowledge, skill competencies, and preparedness in responding to disasters. This review aimed to evaluate nursing preparedness to disasters in terms of knowledge, skill competencies, and psychological preparedness to disasters.
A systematic review was conducted from recent research articles published between 2001 and 2018, which included searches from five databases: PubMed, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Scopus, Medline, and ScienceDirect. Quality of the selected studies was assessed using Mixed Methods Appraisal Tool (MMAT), and the review results were generated through an iterative narrative process of synthesis to identify common themes.
Twelve studies, with a total of 1443 nurses involved, met the inclusion criteria. The articles revealed the need for further development of disaster preparedness of nurses in the aspects of knowledge and skill competencies; and in particular with more focus on the education of nurses to achieve better psychological preparedness.
The results of this review showed that it is important to enhance the psychological preparedness of nurses, in addition to knowledge and skill competencies, so that they can provide the best care possible to affected individuals as well as for themselves.
Abstract Objective The purpose of this study is to evaluate the accuracy rate of the one breath-hold single voxel hydrogen-1 magnetic resonance spectroscopy (MRS) in comparison with intraoperative ...biopsy for liver fat quantification in living-donor liver transplantation. Materials and Methods A total of 80 living liver donors participated in this study. Each patient underwent both MRS and intraoperative biopsy for evaluation of liver fatty content. MRS was performed using 1.5-T magnetic resonance imaging and placed in segments 2–4, 5–8, and left lateral segment for each donor. Accuracy was assessed through receiver operating characteristic curve analysis. Sensitivity and specificity of MRS fat fractions were also calculated. Results Eighty living-donor liver transplantation donors were enrolled in this study. There was no fatty liver in 59 subjects (73.8%), 5% to 10% fatty liver in 17 subjects, 11% to 15% fatty liver in 3 subjects, and >16% fatty liver in 1 subject. MRS fat fraction showed excellent parameters to predict between normal liver and fatty liver groups (1.85% ± 0.98, 8.13% ± 3.52, respectively; P < .0001). Linear regression between MRS fat fraction and pathology grading showed high correlation (R2 = 0.7092). Pearson correlation revealed high correlation between MRS and pathology results ( r = 0.936), poor correlation between body mass index and pathology results ( r = 0.390). The sensitivity and specificity for detection of liver steatosis in MRS fat fraction were 95.2% and 98.3%, respectively. Conclusion1 H MRS fat fraction is a highly precise and accurate method in quantification of hepatic steatosis for the living donor and can be finished in a single breath-hold.
We present measurements of the E-mode polarization angular auto-power spectrum (EE) and temperature-E-mode cross-power spectrum (TE) of the cosmic microwave background (CMB) using 150 GHz data from ...three seasons of SPTpol observations. We report the power spectra over the spherical harmonic multipole range and detect nine acoustic peaks in the EE spectrum with high signal-to-noise ratio. These measurements are the most sensitive to date of the EE and TE power spectra at and , respectively. The observations cover 500 , a fivefold increase in area compared to previous SPTpol analyses, which increases our sensitivity to the photon diffusion damping tail of the CMB power spectra enabling tighter constraints on ΛCDM model extensions. After masking all sources with unpolarized flux mJy, we place a 95% confidence upper limit on residual polarized point-source power of at , suggesting that the EE damping tail dominates foregrounds to at least with modest source masking. We find that the SPTpol data set is in mild tension with the ΛCDM model ( ), and different data splits prefer parameter values that differ at the level. When fitting SPTpol data at , we find cosmological parameter constraints consistent with those for Planck temperature. Including SPTpol data at results in a preference for a higher value of the expansion rate ( ) and a lower value for present-day density fluctuations ( ).
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