Urban waste is a complex mixture of different substances, including microplastics and pharmaceuticals and personal care products. Microplastics have a high affinity for hydrophobic substances. One of ...these substances is triclosan, a bactericide used in a variety of hygiene products. Therefore, microplastics (MPs) may serve as a vector between triclosan and aquatic organisms. The current study sought to evaluate the effects of the interaction between microplastics and triclosan based on a mechanistic approach in which the oyster
Crassostrea brasiliana
was used as a model. The organisms were exposed to three conditions: the control, microplastic (MP), and microplastic contaminated with triclosan (MPT). The organisms were exposed for 3 or 7 days. After the exposure time, hemolymph was sampled for performing the neutral red retention time assay and, subsequently, the gills, digestive glands, and adductor muscles were dissected for measuring biomarkers responses (EROD, DBF, GST, GPx, GSH, lipid peroxidation, DNA strand breaks, and AChE). Our results demonstrate combined effects of MPs associated with triclosan on oyster physiology and biochemistry, as well as on lysosomal membrane stability. These results contribute to understanding the effects of contaminants of emerging concern and microplastics on aquatic organisms.
The major fire at the fuel storage tanks of a Chemical Terminal in April 2015 at the Port of Santos (SP), SE Brazil, potentially caused significant environmental impacts to the Santos Estuarine ...System (SES). The aim of the study was to identify the classes of substances causing pore water toxicity in the SES sediments in the vicinity of the port terminal before and after the incident using the TIE approach. Our results suggest that nonpolar compounds (e.g. PAHs, BTX, oil and grease) from the storage tanks that burned, and surfactants present in the formulation of foam fire extinguishing agents were the responsible for the sediment toxicity observed in the vicinity of the incident site. The contribution of metals to the toxicity related to the incident was ruled out. A long-term monitoring of the environmental effects of the incident is recommended since substances for which evidence of toxicity was found are toxic, persistent and can accumulate in the biota.
Studies which showed the influence of organic carbon on the toxicity of sediment-associated contaminants on benthic invertebrates suggest this was primarily due to its influence on the interstitial ...water concentrations of the contaminant. A higher organic content offers more binding sites for organic contaminants, which means lower toxicity for organisms whose exposure route is mainly through contaminated interstitial water. However, a higher organic content in the sediment could mean a higher toxicity for deposit-feeding organisms, which can assimilate the contaminant by ingestion of contaminated particles. To investigate the influence of sedimentary organic carbon content on the toxicity of an organic contaminant on a benthic community, a microcosm experiment was carried out where natural nematode assemblages were exposed to three concentrations of Irgarol in sediments with two different levels of organic carbon for 7 and 35 days. The response of the nematode assemblage to sediment contamination by Irgarol differed between organically “Lower organic carbon” and “Higher organic carbon” sediments. Responses were genus specific and although community composition was the same in both sediments in the beginning of the assay, contamination by Irgarol affected different genera at each sediment type. Also, the differential amount of organic carbon promoted responses of different functional groups. In Lower organic carbon sediments, contaminated treatments showed lower abundances of the genus Viscosia and the group of predacious nematodes, which were probably affected by an increased availability of Irgarol in the interstitial water in this treatment. In Higher organic carbon sediments, the group of deposit-feeders were mainly affected, suggesting the ingestion of contaminated food as the main route of contamination in this condition. These results indicate that the bioavailability of toxic substances in sediments is not only determined by their partitioning between the different phases of the sediment but also by the organism’s trophic ecology.
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•Sedimentary organic carbon influences the toxicity of Irgarol.•Irgarol affected different nematode genera at sediments with different carbon content.•Food was the main route of contamination to deposit-feeders in organic-rich sediments.•Porewater was the main route of contamination to predators in organic-poor sediments.•Contaminant sediment partitioning and organism’s ecology regulate bioavailability.
Influence of sediment organic carbon on toxicity depends on organism’s trophic ecology.
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•Cigarette butts (CBs) were among the main marine litter on beaches of Santos.•A total of 22 cigarettes brands were found in different stages of decomposition.•CBs can reach sediment ...layers after few days in water column.•Minimal amounts of CBs in leachate induce harmful effects on euryhaline copepods.•Approaches adopted can serve to estimate local impacts of CBs on coastal areas.
Cigarette butts (CBs) comprise one of the most relevant categories of marine litter worldwide. This study was structured as a case study simultaneously assessing (i) the occurrence of CBs in a highly urbanized coastal area, (ii) their partitioning in two environmental compartments (water column and sediments) by laboratory settling tests, and (iii) the toxicity produced by the CBs leachates (measured as copepod reproduction). Marine litter was sampled in beaches of the city of Santos (SW Brazil) and CBs were collected for analysis. The characterization showed that CBs were one of the most prevalent items (51.5% in summer and 34.4% in winter) and 22 cigarettes brands were identified at different stages of decomposition. Laboratory settling tests showed that CBs remain in the water column between 3 and 20 days, subsequently reaching the sediments. The toxicity results indicated that CBs the leachates extracted from a small amount of CBs was sufficient to affect copepod reproduction (0.1 and 0.01 CBs L−1). Furthermore, the approaches adopted by the present study can serve as a preliminary assessment to estimate local impacts resulting from inadequate disposal of cigarette butts in coastal areas around the world.
The gastrointestinal tract (GIT) of fish is a target of contaminants since it can absorb these substances. We evaluated the morphophysiological alterations in the GIT of Sphoeroides testudineus ...collected in two estuaries presenting differences in their environmental quality (NIA and IA). The intestine was analyzed for histological and neuronal changes; liver and gills for biochemical markers; muscle tissues for neurotoxicity and peripheral blood for genotoxic damage. The results showed alterations in the GIT of the animals collected in the IA, such as muscle tunica and goblet cell density reduction, increased intraepithelial lymphocytes density and changes in neuronal density. Furthermore, changes were observed in MTs and LPO in the gills. Thus, we suggest that TGI is functioning as a barrier that responds to ingested contaminants, in order to reduce their absorption and translocation. Thus, alterations in morphophysiological and enteric neurons in S. testudineus can be used as biomarkers of environmental contamination.
•Animals from anthropic environments presented alterations in the intestinal layers.•Contaminated luminal content changes the chemical code of myenteric neurons.•The biomarkers matrix differentiated the estuaries by the PERMANOVA analysis•The enteric nervous system proved to be a potential biomarker.
Estuaries in the world are affected by different contamination sources related to urbanisation and port/industrial activities. Identifying the substances responsible for the environmental toxicity in ...estuaries is challenging due to the multitude of stressors, both natural and anthropogenic. The Toxicity Identification and Evaluation (TIE) is a suitable way of determining causes of toxicity of sediments, but it poses difficulties since its application is labour intensive and time consuming. The aim of this study is to evaluate the diagnosis provided by a TIE based on microscale embryotoxicity tests with interstitial water (IW) to identify toxicants in estuarine sediments affected by multiple stressors. TIE showed toxicity due to different combinations of metals, apolar organic compounds, ammonia and sulphides, depending on the contamination source closest to the sampling station. The microscale TIE was able to discern different toxicants on sites subject to different contamination sources. There is good agreement between the results indicated in the TIE and the chemical analyses in whole sediment, although there are some disagreements, either due to the sensitivity of the test used, or due to the particularities of the use of interstitial water to assess the sediment toxicity. The improvement of TIE methods focused on identifying toxicants in multiple-stressed estuarine areas are crucial to discern contamination sources and subsidise management strategies.
The synthetic hormone 17α ethinyl estradiol (EE2) is a molecule widely used in female contraceptives and recognized as a contaminant of attention (Watch List) in the European Union due to its high ...consumption, endocrine effects and occurrence in aquatic environments. Its main source of introduction is domestic sewage where it can be associated with other contaminants such as microplastics (MPs). Due to their characteristics, they can combine with each other and exacerbate their isolated effects on biota. This study evaluated the combined effects of microplastics (MPs) and 17α ethinylestradiol (EE2) on two tropical estuarine invertebrate species:
and
. Polyethylene particles were spiked with EE2 and organisms were exposed to three treatments, categorized into three groups: control group (C), virgin microplastics (MPs), and spiked microplastics with EE2 (MPEs). All treatments were evaluated after 3 and 7 days of exposure. Oysters exhibited changes in phase 2 enzymes and the antioxidant system, oxidative stress in the gills, and reduced lysosomal membrane stability after exposure to MPs and MPEs. Crabs exposed to MPs and MPEs after seven days showed changes in phase 1 enzymes in the gills and changes in phases 1 and 2 enzymes in the hepatopancreas, such as disturbed cellular health. The combined effects of microplastics and EE2 increased the toxicity experienced by organisms, which may trigger effects at higher levels of biological organization, leading to ecological disturbances in tropical coastal ecosystems.
The present study is a literature-based analysis investigating occurrence and the possible consequences of polycyclic aromatic hydrocarbons (PAH) in marine protected areas (MPAs) of Latin America and ...Caribbean. The approach using overlapping of georeferenced MPA polygons with data compiled from peer-reviewed literature, published during the last 15 years, showed 341 records of PAH in 9 countries. PAH was reported to occur within the boundaries of 36 MPAs located in Argentina, Brazil, Colombia, Mexico, Nicaragua and Uruguay. According to quality guidelines, low to moderate impacts are expected in MPAs categorized in different management classes. Considering sediment samples, 13% of the records presented concentrations enough to cause occasional toxicity. Such level of risk was also seen in Ramsar sites and in Amazonian MPAs. In addition, based on concentrations reported in biota, occasional deleterious effects on organisms from Biosphere Reserves might occur. Diagnostic ratios pointed out petrogenic and pyrolytic processes as PAH predominant sources, and were mainly attributed to the proximity to ports, industries and urban areas. MPAs located in the vicinity of impact-generating areas may be under threat and require government attention and action, mainly through implementation of contamination monitoring programs.
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•PAH occurrence was seen by 341 records in 36 MPA of Latin America and Caribbean.•Affected MPA were seen in Argentina, Brazil, Colombia, Mexico, Nicaragua and Uruguay.•Low to moderate effects upon organisms were suggested to occur in such MPA.•Petrogenic and pyrolytic processes were predominant sources.•Conservancy goals might be under threat in Latin America.
The conservancy goals of MPA from Latin America and Caribbean, located in the vicinity of impact-generating areas, are under threat due to PAH contamination.
The gastrointestinal tract and its enteric nervous system are the first routes of food and xenobiotics uptake. Considering the importance of this organ, this study evaluated intestinal biomarkers of ...Sphoeroides testudineus integrating the data to generate tools for pollution assessment. The fish were collected in three sites of São Paulo Coast and their intestines were analyzed for biochemical, histology, and neuronal density and morphometry biomarkers. To evaluate the differences among the data, a PERMANOVA was applied, followed by a FA/PCA. The PERMANOVA indicated differences (P < 0.001) between the regions (RA, A1, and A2). Four factors were extracted from the FA/PCA (62% cumulative), showing that the animals from A2 presented severe alterations, mainly in intestinal morphometry and neuronal density. A1 alterations refer mainly to the increase of neuronal metabolism. Our results also evidence a gradient of environmental quality related to the protection level (AR > A1 > A2).
•Ambiental stressors caused increase in intestinal layers and IELs.•Stressors disponibles in water can decrease, generally, the density of enteric nervous system neurons.•Areas with high levels of anthropogenic influences can lead to changes in intestinal peristalsis.
